The complete sequence of a heterochromatic island from a higher eukaryote

W. Richard McCombie; Melissa De la Bastide; Kristina Habermann; Laurence Parnell; Neilay Dedhia; Lidia Gnoj; Kristin Schutz; Emily Huang; Lori Spiegel; Cristy Yordan; Mundeep Sehkon; Jennifer Murray; Paul Sheet; Matt Cordes; Jane Threideh; Tamberlyn Stoneking; Joelle Kalicki; Tina Graves; Gwen Harmon; Jennifer Edwards; Phil Latreille; Laura Courtney; James Cloud; Amanda Abbott; Kelsi Scott; Doug Johnson; Pat Minx; Dan Bentley; Bob Fulton; Nancy Miller; Tracie Greco; Kim Kemp; Jason Kramer; Lucinda Fulton; Elaine Mardis; Mike Dante; Kym Pepin; La Deana Hillier; Joanne Nelson; John Spieth; Joe Simorowski; Bruce May; Peter Ma; Ray Preston; Daniel Vil; Hoon See Lei; Monica Shekher; Anthony Matero; Ravi Shah; I'Kyori Swaby; Andrew O'Shaughnessy; Milka Rodriguez; Jane Hoffman; Sally Till; Susan Granat; Nadim Shohdy; Amy Hasegawa; Aliyah Hameed; Mohammad Lodhi; Arthur Johnson; Ellson Chen; Marco Marra; Richard K. Wilson; Robert Martienssen

doi:10.1016/S0092-8674(00)80673-X

The complete sequence of a heterochromatic island from a higher eukaryote

W. Richard McCombie, Melissa De la Bastide, Kristina Habermann, Laurence Parnell, Neilay Dedhia, Lidia Gnoj, Kristin Schutz, Emily Huang, Lori Spiegel, Cristy Yordan, Mundeep Sehkon, Jennifer Murray, Paul Sheet, Matt Cordes, Jane Threideh, Tamberlyn Stoneking, Joelle Kalicki, Tina Graves, Gwen Harmon, Jennifer EdwardsPhil Latreille, Laura Courtney, James Cloud, Amanda Abbott, Kelsi Scott, Doug Johnson, Pat Minx, Dan Bentley, Bob Fulton, Nancy Miller, Tracie Greco, Kim Kemp, Jason Kramer, Lucinda Fulton, Elaine Mardis, Mike Dante, Kym Pepin, La Deana Hillier, Joanne Nelson, John Spieth, Joe Simorowski, Bruce May, Peter Ma, Ray Preston, Daniel Vil, Hoon See Lei, Monica Shekher, Anthony Matero, Ravi Shah, I'Kyori Swaby, Andrew O'Shaughnessy, Milka Rodriguez, Jane Hoffman, Sally Till, Susan Granat, Nadim Shohdy, Amy Hasegawa, Aliyah Hameed, Mohammad Lodhi, Arthur Johnson, Ellson Chen, Marco Marra, Richard K. Wilson, Robert Martienssen

McDonnell Genome Institute (MGI)

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

Heterochromatin, constitutively condensed chromosomal material, is widespread among eukaryotes but incompletely characterized at the nucleotide level. We have sequenced and analyzed 2.1 megabases (Mb) of Arabidopsis thaliana chromosome 4 that includes 0.5-0.7 Mb of isolated heterochromatin that resembles the chromosomal knobs described by Barbara McClintock in maize. This isolated region has a low density of expressed genes, low levels of recombination and a low incidence of genetrap insertion. Satellite repeats were absent, but tandem arrays of long repeats and many transposons were found. Methylation of these sequences was dependent on chromatin remodeling. Clustered repeats were associated with condensed chromosomal domains elsewhere. The complete sequence of a heterochromatic island provides an opportunity to study sequence determinants of chromosome condensation.

Original language	English
Pages (from-to)	377-386
Number of pages	10
Journal	Cell
Volume	100
Issue number	3
DOIs	https://doi.org/10.1016/S0092-8674(00)80673-X
State	Published - Feb 4 2000

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McCombie, W. R., De la Bastide, M., Habermann, K., Parnell, L., Dedhia, N., Gnoj, L., Schutz, K., Huang, E., Spiegel, L., Yordan, C., Sehkon, M., Murray, J., Sheet, P., Cordes, M., Threideh, J., Stoneking, T., Kalicki, J., Graves, T., Harmon, G., ... Martienssen, R. (2000). The complete sequence of a heterochromatic island from a higher eukaryote. Cell, 100(3), 377-386. https://doi.org/10.1016/S0092-8674(00)80673-X

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title = "The complete sequence of a heterochromatic island from a higher eukaryote",

abstract = "Heterochromatin, constitutively condensed chromosomal material, is widespread among eukaryotes but incompletely characterized at the nucleotide level. We have sequenced and analyzed 2.1 megabases (Mb) of Arabidopsis thaliana chromosome 4 that includes 0.5-0.7 Mb of isolated heterochromatin that resembles the chromosomal knobs described by Barbara McClintock in maize. This isolated region has a low density of expressed genes, low levels of recombination and a low incidence of genetrap insertion. Satellite repeats were absent, but tandem arrays of long repeats and many transposons were found. Methylation of these sequences was dependent on chromatin remodeling. Clustered repeats were associated with condensed chromosomal domains elsewhere. The complete sequence of a heterochromatic island provides an opportunity to study sequence determinants of chromosome condensation.",

author = "McCombie, {W. Richard} and {De la Bastide}, Melissa and Kristina Habermann and Laurence Parnell and Neilay Dedhia and Lidia Gnoj and Kristin Schutz and Emily Huang and Lori Spiegel and Cristy Yordan and Mundeep Sehkon and Jennifer Murray and Paul Sheet and Matt Cordes and Jane Threideh and Tamberlyn Stoneking and Joelle Kalicki and Tina Graves and Gwen Harmon and Jennifer Edwards and Phil Latreille and Laura Courtney and James Cloud and Amanda Abbott and Kelsi Scott and Doug Johnson and Pat Minx and Dan Bentley and Bob Fulton and Nancy Miller and Tracie Greco and Kim Kemp and Jason Kramer and Lucinda Fulton and Elaine Mardis and Mike Dante and Kym Pepin and Hillier, {La Deana} and Joanne Nelson and John Spieth and Joe Simorowski and Bruce May and Peter Ma and Ray Preston and Daniel Vil and Lei, {Hoon See} and Monica Shekher and Anthony Matero and Ravi Shah and I'Kyori Swaby and Andrew O'Shaughnessy and Milka Rodriguez and Jane Hoffman and Sally Till and Susan Granat and Nadim Shohdy and Amy Hasegawa and Aliyah Hameed and Mohammad Lodhi and Arthur Johnson and Ellson Chen and Marco Marra and Wilson, {Richard K.} and Robert Martienssen",

note = "Funding Information: We would like to thank Eric Richards for comments on the manuscript and for ddm1 seed. We would like to thank Bruce May, John Healy, and Andy Reiner for help with the genetrap sequence analysis, and Juana-Marie Arroyo and Rulan Shen for sharing unpublished genetrap data. We acknowledge the support of the United States Department of Agriculture NRI (95-37300-1578), the National Science Foundation (DBI-9813578), the Robertson Research Fund, Westvaco Corporation, and the generous support of David L. Luke III. ",

year = "2000",

month = feb,

day = "4",

doi = "10.1016/S0092-8674(00)80673-X",

language = "English",

volume = "100",

pages = "377--386",

journal = "Cell",

issn = "0092-8674",

number = "3",

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McCombie, WR, De la Bastide, M, Habermann, K, Parnell, L, Dedhia, N, Gnoj, L, Schutz, K, Huang, E, Spiegel, L, Yordan, C, Sehkon, M, Murray, J, Sheet, P, Cordes, M, Threideh, J, Stoneking, T, Kalicki, J, Graves, T, Harmon, G, Edwards, J, Latreille, P, Courtney, L, Cloud, J, Abbott, A, Scott, K, Johnson, D, Minx, P, Bentley, D, Fulton, B, Miller, N, Greco, T, Kemp, K, Kramer, J, Fulton, L, Mardis, E, Dante, M, Pepin, K, Hillier, LD, Nelson, J, Spieth, J, Simorowski, J, May, B, Ma, P, Preston, R, Vil, D, Lei, HS, Shekher, M, Matero, A, Shah, R, Swaby, IK, O'Shaughnessy, A, Rodriguez, M, Hoffman, J, Till, S, Granat, S, Shohdy, N, Hasegawa, A, Hameed, A, Lodhi, M, Johnson, A, Chen, E, Marra, M, Wilson, RK & Martienssen, R 2000, 'The complete sequence of a heterochromatic island from a higher eukaryote', Cell, vol. 100, no. 3, pp. 377-386. https://doi.org/10.1016/S0092-8674(00)80673-X

TY - JOUR

T1 - The complete sequence of a heterochromatic island from a higher eukaryote

AU - McCombie, W. Richard

AU - De la Bastide, Melissa

AU - Habermann, Kristina

AU - Parnell, Laurence

AU - Dedhia, Neilay

AU - Gnoj, Lidia

AU - Schutz, Kristin

AU - Huang, Emily

AU - Spiegel, Lori

AU - Yordan, Cristy

AU - Sehkon, Mundeep

AU - Murray, Jennifer

AU - Sheet, Paul

AU - Cordes, Matt

AU - Threideh, Jane

AU - Stoneking, Tamberlyn

AU - Kalicki, Joelle

AU - Graves, Tina

AU - Harmon, Gwen

AU - Edwards, Jennifer

AU - Latreille, Phil

AU - Courtney, Laura

AU - Cloud, James

AU - Abbott, Amanda

AU - Scott, Kelsi

AU - Johnson, Doug

AU - Minx, Pat

AU - Bentley, Dan

AU - Fulton, Bob

AU - Miller, Nancy

AU - Greco, Tracie

AU - Kemp, Kim

AU - Kramer, Jason

AU - Fulton, Lucinda

AU - Mardis, Elaine

AU - Dante, Mike

AU - Pepin, Kym

AU - Hillier, La Deana

AU - Nelson, Joanne

AU - Spieth, John

AU - Simorowski, Joe

AU - May, Bruce

AU - Ma, Peter

AU - Preston, Ray

AU - Vil, Daniel

AU - Lei, Hoon See

AU - Shekher, Monica

AU - Matero, Anthony

AU - Shah, Ravi

AU - Swaby, I'Kyori

AU - O'Shaughnessy, Andrew

AU - Rodriguez, Milka

AU - Hoffman, Jane

AU - Till, Sally

AU - Granat, Susan

AU - Shohdy, Nadim

AU - Hasegawa, Amy

AU - Hameed, Aliyah

AU - Lodhi, Mohammad

AU - Johnson, Arthur

AU - Chen, Ellson

AU - Marra, Marco

AU - Wilson, Richard K.

AU - Martienssen, Robert

N1 - Funding Information: We would like to thank Eric Richards for comments on the manuscript and for ddm1 seed. We would like to thank Bruce May, John Healy, and Andy Reiner for help with the genetrap sequence analysis, and Juana-Marie Arroyo and Rulan Shen for sharing unpublished genetrap data. We acknowledge the support of the United States Department of Agriculture NRI (95-37300-1578), the National Science Foundation (DBI-9813578), the Robertson Research Fund, Westvaco Corporation, and the generous support of David L. Luke III.

PY - 2000/2/4

Y1 - 2000/2/4

N2 - Heterochromatin, constitutively condensed chromosomal material, is widespread among eukaryotes but incompletely characterized at the nucleotide level. We have sequenced and analyzed 2.1 megabases (Mb) of Arabidopsis thaliana chromosome 4 that includes 0.5-0.7 Mb of isolated heterochromatin that resembles the chromosomal knobs described by Barbara McClintock in maize. This isolated region has a low density of expressed genes, low levels of recombination and a low incidence of genetrap insertion. Satellite repeats were absent, but tandem arrays of long repeats and many transposons were found. Methylation of these sequences was dependent on chromatin remodeling. Clustered repeats were associated with condensed chromosomal domains elsewhere. The complete sequence of a heterochromatic island provides an opportunity to study sequence determinants of chromosome condensation.

AB - Heterochromatin, constitutively condensed chromosomal material, is widespread among eukaryotes but incompletely characterized at the nucleotide level. We have sequenced and analyzed 2.1 megabases (Mb) of Arabidopsis thaliana chromosome 4 that includes 0.5-0.7 Mb of isolated heterochromatin that resembles the chromosomal knobs described by Barbara McClintock in maize. This isolated region has a low density of expressed genes, low levels of recombination and a low incidence of genetrap insertion. Satellite repeats were absent, but tandem arrays of long repeats and many transposons were found. Methylation of these sequences was dependent on chromatin remodeling. Clustered repeats were associated with condensed chromosomal domains elsewhere. The complete sequence of a heterochromatic island provides an opportunity to study sequence determinants of chromosome condensation.

UR - http://www.scopus.com/inward/record.url?scp=0034603217&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)80673-X

DO - 10.1016/S0092-8674(00)80673-X

M3 - Article

C2 - 10676819

AN - SCOPUS:0034603217

SN - 0092-8674

VL - 100

SP - 377

EP - 386

JO - Cell

JF - Cell

IS - 3

ER -

The complete sequence of a heterochromatic island from a higher eukaryote

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