The coding and long noncoding single-cell atlas of the developing human fetal striatum

Vittoria Dickinson Bocchi; Paola Conforti; Elena Vezzoli; Dario Besusso; Claudio Cappadona; Tiziana Lischetti; Maura Galimberti; Valeria Ranzani; Raoul J.P. Bonnal; Marco De Simone; Grazisa Rossetti; Xiaoling He; Kenji Kamimoto; Ira Espuny-Camacho; Andrea Faedo; Federica Gervasoni; Romina Vuono; Samantha A. Morris; Jian Chen; Dan Felsenfeld; Giulio Pavesi; Roger A. Barker

doi:10.1126/science.abf5759

The coding and long noncoding single-cell atlas of the developing human fetal striatum

Vittoria Dickinson Bocchi, Paola Conforti, Elena Vezzoli, Dario Besusso, Claudio Cappadona, Tiziana Lischetti, Maura Galimberti, Valeria Ranzani, Raoul J.P. Bonnal, Marco De Simone, Grazisa Rossetti, Xiaoling He, Kenji Kamimoto, Ira Espuny-Camacho, Andrea Faedo, Federica Gervasoni, Romina Vuono, Samantha A. Morris, Jian Chen, Dan FelsenfeldGiulio Pavesi, Roger A. Barker

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Deciphering how the human striatum develops is necessary for understanding the diseases that affect this region. To decode the transcriptional modules that regulate this structure during development, we compiled a catalog of 1116 long intergenic noncoding RNAs (lincRNAs) identified de novo and then profiled 96,789 single cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (D1- A nd D2-MSNs) arise from a common progenitor and that lineage commitment is established during the postmitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type-specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. This work delineates the cellular hierarchies governing MSN lineage commitment.

Original language	English
Article number	abf5759
Journal	Science
Volume	372
Issue number	6542
DOIs	https://doi.org/10.1126/science.abf5759
State	Published - May 7 2021

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Bocchi, V. D., Conforti, P., Vezzoli, E., Besusso, D., Cappadona, C., Lischetti, T., Galimberti, M., Ranzani, V., Bonnal, R. J. P., Simone, M. D., Rossetti, G., He, X., Kamimoto, K., Espuny-Camacho, I., Faedo, A., Gervasoni, F., Vuono, R., Morris, S. A., Chen, J., ... Barker, R. A. (2021). The coding and long noncoding single-cell atlas of the developing human fetal striatum. Science, 372(6542), Article abf5759. https://doi.org/10.1126/science.abf5759

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title = "The coding and long noncoding single-cell atlas of the developing human fetal striatum",

abstract = "Deciphering how the human striatum develops is necessary for understanding the diseases that affect this region. To decode the transcriptional modules that regulate this structure during development, we compiled a catalog of 1116 long intergenic noncoding RNAs (lincRNAs) identified de novo and then profiled 96,789 single cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (D1- A nd D2-MSNs) arise from a common progenitor and that lineage commitment is established during the postmitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type-specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. This work delineates the cellular hierarchies governing MSN lineage commitment.",

author = "Bocchi, {Vittoria Dickinson} and Paola Conforti and Elena Vezzoli and Dario Besusso and Claudio Cappadona and Tiziana Lischetti and Maura Galimberti and Valeria Ranzani and Bonnal, {Raoul J.P.} and Simone, {Marco De} and Grazisa Rossetti and Xiaoling He and Kenji Kamimoto and Ira Espuny-Camacho and Andrea Faedo and Federica Gervasoni and Romina Vuono and Morris, {Samantha A.} and Jian Chen and Dan Felsenfeld and Giulio Pavesi and Barker, {Roger A.}",

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doi = "10.1126/science.abf5759",

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Bocchi, VD, Conforti, P, Vezzoli, E, Besusso, D, Cappadona, C, Lischetti, T, Galimberti, M, Ranzani, V, Bonnal, RJP, Simone, MD, Rossetti, G, He, X, Kamimoto, K, Espuny-Camacho, I, Faedo, A, Gervasoni, F, Vuono, R, Morris, SA, Chen, J, Felsenfeld, D, Pavesi, G & Barker, RA 2021, 'The coding and long noncoding single-cell atlas of the developing human fetal striatum', Science, vol. 372, no. 6542, abf5759. https://doi.org/10.1126/science.abf5759

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T1 - The coding and long noncoding single-cell atlas of the developing human fetal striatum

AU - Bocchi, Vittoria Dickinson

AU - Conforti, Paola

AU - Vezzoli, Elena

AU - Besusso, Dario

AU - Cappadona, Claudio

AU - Lischetti, Tiziana

AU - Galimberti, Maura

AU - Ranzani, Valeria

AU - Bonnal, Raoul J.P.

AU - Simone, Marco De

AU - Rossetti, Grazisa

AU - He, Xiaoling

AU - Kamimoto, Kenji

AU - Espuny-Camacho, Ira

AU - Faedo, Andrea

AU - Gervasoni, Federica

AU - Vuono, Romina

AU - Morris, Samantha A.

AU - Chen, Jian

AU - Felsenfeld, Dan

AU - Pavesi, Giulio

AU - Barker, Roger A.

PY - 2021/5/7

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AB - Deciphering how the human striatum develops is necessary for understanding the diseases that affect this region. To decode the transcriptional modules that regulate this structure during development, we compiled a catalog of 1116 long intergenic noncoding RNAs (lincRNAs) identified de novo and then profiled 96,789 single cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (D1- A nd D2-MSNs) arise from a common progenitor and that lineage commitment is established during the postmitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type-specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. This work delineates the cellular hierarchies governing MSN lineage commitment.

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VL - 372

JO - Science

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ER -

The coding and long noncoding single-cell atlas of the developing human fetal striatum

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