TY - JOUR
T1 - The cloning and developmental regulation of murine diacylglycerol kinase ζ
AU - Ding, Li
AU - McIntyre, Thomas M.
AU - Zimmerman, Guy A.
AU - Prescott, Stephen M.
N1 - Funding Information:
We thank Matt Topham for many suggestions and discussions, Elie Traer for the sequence analysis, and Diana Lim for the preparation of figures. Susan Mango and Diana Stafforini contributed helpful comments on the manuscript. This work was supported by Grant CA59548 from the National Cancer Institute. The DNA sequencing core facility at the University of Utah is supported by Grant CA42014 from the National Cancer Institute.
PY - 1998/6/5
Y1 - 1998/6/5
N2 - Diacylglycerol kinases (DGKs) regulate the key signaling intermediates diacylglycerol (DAG) and phosphatidic acid (PA). We isolated cDNA clones of mouse diacylglycerol kinase ζ (mDGKζ) and found that it shares 88% identify at the nucleic acid level and 95.5% identity at the amino acid level with human DGKζ (hDGKζ). Murine DGKζ protein rose gradually during embryonic development, and was abundant in newborn and adult brains. By RNA whole- mount in situ hybridization, mDGKζ was shown to be expressed in spinal ganglia and limb buds at low level in E11.5 embryos and at higher level in E12.5 embryos. In E13.5 embryos, DGKζ mRNA was highly expressed in vibrissa follicles, in spinal ganglia, and in the interdigital regions of the developing limbs. Northern blotting showed that DGKζ expression was limited to specific anatomical regions of the brain. Thus, the expression of DGKζ is regulated temporally and spatially during mammalian development and correlates with the development of sensory neurons and regions undergoing apoptosis.
AB - Diacylglycerol kinases (DGKs) regulate the key signaling intermediates diacylglycerol (DAG) and phosphatidic acid (PA). We isolated cDNA clones of mouse diacylglycerol kinase ζ (mDGKζ) and found that it shares 88% identify at the nucleic acid level and 95.5% identity at the amino acid level with human DGKζ (hDGKζ). Murine DGKζ protein rose gradually during embryonic development, and was abundant in newborn and adult brains. By RNA whole- mount in situ hybridization, mDGKζ was shown to be expressed in spinal ganglia and limb buds at low level in E11.5 embryos and at higher level in E12.5 embryos. In E13.5 embryos, DGKζ mRNA was highly expressed in vibrissa follicles, in spinal ganglia, and in the interdigital regions of the developing limbs. Northern blotting showed that DGKζ expression was limited to specific anatomical regions of the brain. Thus, the expression of DGKζ is regulated temporally and spatially during mammalian development and correlates with the development of sensory neurons and regions undergoing apoptosis.
KW - Diacylglycerol kinase
KW - Distribution
KW - Embryonic development
UR - http://www.scopus.com/inward/record.url?scp=0345214535&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00490-6
DO - 10.1016/S0014-5793(98)00490-6
M3 - Article
C2 - 9657393
AN - SCOPUS:0345214535
SN - 0014-5793
VL - 429
SP - 109
EP - 114
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -