The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation

Felipe A. Pinho-Ribeiro, Ana C. Zarpelon, Sandra S. Mizokami, Sergio M. Borghi, Juliano Bordignon, Rangel L. Silva, Thiago M. Cunha, Jose C. Alves-Filho, Fernando Q. Cunha, Rubia Casagrande, Waldiceu A. Verri

Research output: Contribution to journalArticlepeer-review

101 Scopus citations

Abstract

Lipopolysaccharide (LPS) is the major structural component of Gram-negative bacteria cell wall and a highly pro-inflammatory toxin. Naringenin is found in Citrus fruits and exhibits antioxidant and anti-inflammatory properties through inhibition of NF-κB activation but its effects in LPS-induced inflammatory pain and leukocyte recruitment were not investigated yet. We investigated the effects of naringenin in mechanical hyperalgesia, thermal hyperalgesia and leukocyte recruitment induced by intraplantar injection of LPS in mice. We found that naringenin reduced hyperalgesia to mechanical and thermal stimuli, myeloperoxidase (MPO, a neutrophil and macrophage marker) and N-acetyl-β-D-glucosaminidase (NAG, a macrophage marker) activities, oxidative stress and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production in the paw skin. In the peritoneal cavity, naringenin reduced neutrophil and mononuclear cell recruitment, and abrogated MPO and NAG activity, cytokine and superoxide anion production, and lipid peroxidation. In vitro, pre-treatment with naringenin inhibited superoxide anion and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production by LPS-stimulated RAW 264.7 macrophages. Finally, we demonstrated that naringenin inhibited NF-κB activation in vitro and in vivo. Therefore, naringenin is a promising compound to treat LPS-induced inflammatory pain and leukocyte recruitment.

Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume33
DOIs
StatePublished - Jul 1 2016

Keywords

  • Cytokines
  • Inflammatory pain
  • Leukocyte recruitment
  • NF-κB
  • Oxidative stress

Fingerprint

Dive into the research topics of 'The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation'. Together they form a unique fingerprint.

Cite this