The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone

K. Takebe, M. F. Rai, E. J. Schmidt, L. J. Sandell

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Objective: C-C chemokine receptor type 5 (CCR5) has been implicated in rheumatoid arthritis and several inflammatory diseases, where its blockade resulted in reduced joint destruction. However, its role in modulating cartilage and bone changes in post-traumatic osteoarthritis (OA) has not yet been investigated. In this study, we investigated changes in articular cartilage, synovium and bone in a post-traumatic OA model using CCR5-deficient (CCR5-/-) mice. Method: Destabilization of the medial meniscus (DMM) was performed on the right knee of 10-week old CCR5-/- and C57BL/6J wild-type (WT) mice to induce post-traumatic OA. The contralateral left knee served as sham-operated control. Knee joints were analyzed at 4-, 8- and 12-weeks after surgery to evaluate cartilage degeneration and synovitis by histology, and bone changes via micro-CT. Results: Our findings showed that CCR5-/- mice exhibited significantly less cartilage degeneration than WT mice at 8- and 12-weeks post-surgery. CCR5-/- mice showed some altered bone parameters 18- and 22-weeks of age, but body size and weight were not affected. The effect of CCR5-ablation was insignificant at all time points post-surgery for synovitis and for bone parameters such as bone volume/total volume, connectivity density index (CDI), structure model index (SMI), subchondral bone plate thickness, and trabecular bone number, thickness and spacing. Conclusion: These findings suggest that CCR5-/- mice developed less cartilage degeneration, which may indicate a potential protective role of CCR5-ablation in cartilage homeostasis. There were no differences in bone or synovial response to surgery suggesting that CCR5 functions primarily in cartilage during the development of post-traumatic OA.

Original languageEnglish
Pages (from-to)454-461
Number of pages8
JournalOsteoarthritis and Cartilage
Volume23
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Bone
  • CCR5
  • Cartilage
  • Osteoarthritis
  • Post-traumatic
  • Synovium

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