TY - JOUR
T1 - The canine hookworm genome
T2 - Analysis and classification of Ancylostoma caninum survey sequences
AU - Abubucker, Sahar
AU - Martin, John
AU - Yin, Yong
AU - Fulton, Lucinda
AU - Yang, Shiaw Pyng
AU - Hallsworth-Pepin, Kym
AU - Johnston, J. Spencer
AU - Hawdon, John
AU - McCarter, James P.
AU - Wilson, Richard K.
AU - Mitreva, Makedonka
N1 - Funding Information:
Ancylostoma EST sequencing at Washington University was supported by NIH-NIAID research grant AI 46593 to RKW. JPM is employee and equity holder of Divergence Inc; this research was not company funded.
PY - 2008/2
Y1 - 2008/2
N2 - Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a genome that we estimate by flow cytometry of isolated nuclei to be 347 ± 1.2 Mb, substantially larger than other Rhabditina species. Gene finding identified 5538 genes in the GSS assembly, for a total of 9113 non-redundant A. caninum genes when EST sequences are also considered. Functional classifications of many of the 70% of genes with homology to genes in other species are provided based on gene ontology and KEGG associations and secreted and membrane-bound proteins are also identified.
AB - Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a genome that we estimate by flow cytometry of isolated nuclei to be 347 ± 1.2 Mb, substantially larger than other Rhabditina species. Gene finding identified 5538 genes in the GSS assembly, for a total of 9113 non-redundant A. caninum genes when EST sequences are also considered. Functional classifications of many of the 70% of genes with homology to genes in other species are provided based on gene ontology and KEGG associations and secreted and membrane-bound proteins are also identified.
KW - Ancylostoma caninum
KW - Comparative genomics
KW - Expressed sequence tags
KW - Genome
KW - Genome survey sequences
KW - Hookworm
UR - http://www.scopus.com/inward/record.url?scp=38049107974&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2007.11.001
DO - 10.1016/j.molbiopara.2007.11.001
M3 - Article
C2 - 18082904
AN - SCOPUS:38049107974
SN - 0166-6851
VL - 157
SP - 187
EP - 192
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -