TY - JOUR
T1 - The C4 and Slp genes of the complement region of the murine H-2 major histocompatibility complex.
AU - Shreffler, D. C.
AU - Atkinson, J. P.
AU - Chan, A. C.
AU - Karp, D. R.
AU - Killion, C. C.
AU - Ogata, R. T.
AU - Rosa, P. A.
PY - 1984
Y1 - 1984
N2 - Recent analyses, at the protein and DNA levels of structure, of the murine complement components C4 and the closely related sex-limited protein, Slp have led to new insights into the H-2/S region-linked C4 and Slp genes and their products. The primary products are 200 000 Da precursors which are cleaved, intracellularly and extracellularly, into the the mature alpha-beta-gamma-subunit molecules of plasma. Precursor order of subunits is beta-alpha-gamma; a complementary DNA clone spanning the alpha-gamma junction has been extensively analysed. The C-terminal of the alpha-chain is of particular interest because of post-secretion processing which differentiates 'secreted' and 'plasma' forms of C4, both apparently functional, and because allelic variants of C4 and the Slp protein, which differ substantially in molecular masses, owe their differences principally to different levels of glycosylation of the alpha-chain. Allelic variations in rate of C4 synthesis (C4-high compared with C4-low) have been analysed in cultures of hepatocytes and macrophages. Three distinct modes of genetic regulation of the expression of the Slp protein have been identified.
AB - Recent analyses, at the protein and DNA levels of structure, of the murine complement components C4 and the closely related sex-limited protein, Slp have led to new insights into the H-2/S region-linked C4 and Slp genes and their products. The primary products are 200 000 Da precursors which are cleaved, intracellularly and extracellularly, into the the mature alpha-beta-gamma-subunit molecules of plasma. Precursor order of subunits is beta-alpha-gamma; a complementary DNA clone spanning the alpha-gamma junction has been extensively analysed. The C-terminal of the alpha-chain is of particular interest because of post-secretion processing which differentiates 'secreted' and 'plasma' forms of C4, both apparently functional, and because allelic variants of C4 and the Slp protein, which differ substantially in molecular masses, owe their differences principally to different levels of glycosylation of the alpha-chain. Allelic variations in rate of C4 synthesis (C4-high compared with C4-low) have been analysed in cultures of hepatocytes and macrophages. Three distinct modes of genetic regulation of the expression of the Slp protein have been identified.
UR - http://www.scopus.com/inward/record.url?scp=0021756378&partnerID=8YFLogxK
U2 - 10.1098/rstb.1984.0100
DO - 10.1098/rstb.1984.0100
M3 - Review article
C2 - 6149582
AN - SCOPUS:0021756378
SN - 0962-8436
VL - 306
SP - 395
EP - 403
JO - Philosophical transactions of the Royal Society of London. Series B, Biological sciences
JF - Philosophical transactions of the Royal Society of London. Series B, Biological sciences
IS - 1129
ER -