The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells

Heather A. Pancio, Nancy Vander Heyden, Lee Ratner

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular localization of a GFP-Vpx fusion protein was found to be predominantly nuclear. However, deletion of the proline-rich C-terminal 11 residues of Vpx resulted in a shift of the fusion protein to the cytoplasm. Furthermore, the same deletion in the context of the provirus resulted in a decrease in nuclear import of the preintegration complex and attenuated replication in macrophages.

Original languageEnglish
Pages (from-to)6162-6167
Number of pages6
JournalJournal of virology
Volume74
Issue number13
DOIs
StatePublished - 2000

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