The binding of ORC2 to chromatin from terminally differentiated cells

H. Xu, Z. H. Lu, G. H. Leno

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Nuclei from terminally differentiated Xenopus erythrocytes lack essential components of the pre-replication complex, including the origin recognition complex (ORC) proteins XORC1 and XORC2. In Xenopus egg extract, these proteins are able to bind erythrocyte chromatin from permeable nuclei, but not from intact nuclei, even though they are able to cross an intact nuclear envelope. In this report we use both permeable and intact erythrocyte nuclei to investigate the role of cyclin-dependent kinase activity in modulating the binding of XORC2 to chromatin. We find that elevating the level of cyclin A-dependent kinase in egg extract prevents the binding of XORC2 to chromatin from permeable nuclei and that kinase inhibition reverses this effect. We also observe a nuclear transport-dependent accumulation of H1 kinase activity within intact nuclei incubated in the extract. However, inhibiting this kinase activity does not facilitate the binding of XORC2 to chromatin, suggesting that other molecules and/or mechanisms exist to prevent association of XORC proteins with replication origins within intact nuclei from terminally differentiated cells.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalExperimental Cell Research
Volume274
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Chromatin
  • Cyclin-dependent kinase
  • Erythrocyte nuclei
  • Nuclear envelope
  • Origin recognition complex
  • Terminal differentiation
  • Xenopus egg extract

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