Abstract
The use of hydrogen/deuterium (HD) exchange coupled with mass spectrometry (MS) for the study of the binding of Mg (II) to ras-GDP protein was presented. The H/D exchanged solution was prepared by quenching the stock ras-GDP-Mg solution with decreased pH of 2.6. The complex dissociated to apo-ras, leaving an H/D exchange pattern that represented the complex. the dissociation constant was measured by titration of ras-GDP with Mg 2+.
Original language | English |
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Pages | 301-302 |
Number of pages | 2 |
State | Published - 2002 |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Conference
Conference | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 06/2/02 → 06/6/02 |