The binding of cytochalasin D to monomeric actin has been measured directly. In the presence of 200 μM Ca2+, actin binds cytochalasin D in a 1:1 molar ratio with a KD of 18 μM. After incubation with 250 μM Mg2+ for 10 minutes, actin binds cytochalasin D with a KD of 2.6 μM but with one mole of cytochalasin D per 2 moles of actin. This suggests that cytochalasin D induces dimerization of Mg2+-induced actin monomers.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - May 16 1985


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