The binding of cytochalasin D to monomeric actin has been measured directly. In the presence of 200 μM Ca2+, actin binds cytochalasin D in a 1:1 molar ratio with a KD of 18 μM. After incubation with 250 μM Mg2+ for 10 minutes, actin binds cytochalasin D with a KD of 2.6 μM but with one mole of cytochalasin D per 2 moles of actin. This suggests that cytochalasin D induces dimerization of Mg2+-induced actin monomers.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 16 1985|