The Application of Fluorine-Containing Reagents in Structural Proteomics

Ming Cheng, Chunyang Guo, Michael L. Gross

Research output: Contribution to journalReview articlepeer-review

48 Scopus citations


Structural proteomics refers to large-scale mapping of protein structures in order to understand the relationship between protein sequence, structure, and function. Chemical labeling, in combination with mass-spectrometry (MS) analysis, have emerged as powerful tools to enable a broad range of biological applications in structural proteomics. The key to success is a biocompatible reagent that modifies a protein without affecting its high-order structure. Fluorine, well-known to exert profound effects on the physical and chemical properties of reagents, should have an impact on structural proteomics. In this Minireview, we describe several fluorine-containing reagents that can be applied in structural proteomics. We organize their applications around four MS-based techniques: a) affinity labeling, b) activity-based protein profiling (ABPP), c) protein footprinting, and d) protein cross-linking. Our aim is to provide an overview of the research, development, and application of fluorine-containing reagents in protein structural studies.

Original languageEnglish
Pages (from-to)5880-5889
Number of pages10
JournalAngewandte Chemie - International Edition
Issue number15
StatePublished - Apr 6 2020


  • affinity labeling
  • affinity-based protein profiling
  • mass spectrometry
  • protein cross-linking
  • protein footprinting


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