The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants

Chang Liu, Daming Zhou, Rungtiwa Nutalai, Helen M.E. Duyvesteyn, Aekkachai Tuekprakhon, Helen M. Ginn, Wanwisa Dejnirattisai, Piyada Supasa, Alexander J. Mentzer, Beibei Wang, James Brett Case, Yuguang Zhao, Donal T. Skelly, Rita E. Chen, Sile Ann Johnson, Thomas G. Ritter, Chris Mason, Tariq Malik, Nigel Temperton, Neil G. PatersonMark A. Williams, David R. Hall, Daniel K. Clare, Andrew Howe, Philip J.R. Goulder, Elizabeth E. Fry, Michael S. Diamond, Juthathip Mongkolsapaya, Jingshan Ren, David I. Stuart, Gavin R. Screaton

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the antigenic structure of S, causing escape from natural or vaccine-induced immunity. Beta is particularly difficult to neutralize using serum induced by early pandemic SARS-CoV-2 strains and is most antigenically separated from Delta. To understand this, we generated 674 mAbs from Beta-infected individuals and performed a detailed structure-function analysis of the 27 most potent mAbs: one binding the spike N-terminal domain (NTD), the rest the receptor-binding domain (RBD). Two of these RBD-binding mAbs recognize a neutralizing epitope conserved between SARS-CoV-1 and -2, while 18 target mutated residues in Beta: K417N, E484K, and N501Y. There is a major response to N501Y, including a public IgVH4-39 sequence, with E484K and K417N also targeted. Recognition of these key residues underscores why serum from Beta cases poorly neutralizes early pandemic and Delta viruses.

Original languageEnglish
Pages (from-to)53-68.e12
JournalCell Host and Microbe
Volume30
Issue number1
DOIs
StatePublished - Jan 12 2022

Keywords

  • Beta variant
  • COVID-19
  • SARS-CoV-2
  • antibody
  • immune responses
  • neutralization
  • receptor-binding domain
  • spike protein
  • structure
  • vaccine

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