TY - JOUR
T1 - The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots
AU - Siciliano, Camilla
AU - Bordin, Antonella
AU - Ibrahim, Mohsen
AU - Chimenti, Isotta
AU - Cassiano, Francesco
AU - Gatto, Ilenia
AU - Mangino, Giorgio
AU - Coccia, Andrea
AU - Miglietta, Selenia
AU - Bastianelli, Daniela
AU - Petrozza, Vincenzo
AU - Calogero, Antonella
AU - Frati, Giacomo
AU - De Falco, Elena
N1 - Publisher Copyright:
© 2016 The Authors
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.
AB - Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.
KW - Adipose tissue
KW - Mesenchymal stem cells
KW - Platelet lysate angiogenesis
KW - Transmission electron microscopy
UR - https://www.scopus.com/pages/publications/84986000905
U2 - 10.1016/j.scr.2016.07.010
DO - 10.1016/j.scr.2016.07.010
M3 - Article
C2 - 27614132
AN - SCOPUS:84986000905
SN - 1873-5061
VL - 17
SP - 342
EP - 351
JO - Stem Cell Research
JF - Stem Cell Research
IS - 2
ER -