@article{fed1fb5072a64ed8b43f76e3b30b101d,
title = "The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA",
abstract = "In the vertebrate central nervous system, myelinating oligodendrocytes are postmitotic and derive from proliferative oligodendrocyte precursor cells (OPCs). The molecular mechanisms that govern oligodendrocyte development are incompletely understood, but recent studies implicate the adhesion class of G protein-coupled receptors (aGPCRs) as important regulators of myelination. Here, we use zebrafish and mouse models to dissect the function of the aGPCR Gpr56 in oligodendrocyte development. We show that gpr56 is expressed during early stages of oligodendrocyte development. In addition, we observe a significant reduction of mature oligodendrocyte number and myelinated axons in gpr56 zebrafish mutants. This reduction results from decreased OPC proliferation, rather than increased cell death or altered neural precursor differentiation potential. Finally, we show that these functions are mediated by Gα12/13 proteins and Rho activation. Together, our data establish Gpr56 as a regulator of oligodendrocyte development.",
author = "Ackerman, {Sarah D.} and Cynthia Garcia and Xianhua Piao and Gutmann, {David H.} and Monk, {Kelly R.}",
note = "Funding Information: We thank Lila Solnica-Krezel, members of the Monk laboratory, and the Washington University GPCR group for critical comments on the data and manuscript. We thank Jimann Shin for assistance designing TALENs and Renate Lewis for constructing TALENs. We thank Mark Voigt (St. Louis University) for the generous gift of the tg(sox10( − 7.2):memGFP) fish, Fang Lin (University of Iowa) for morpholinos, the Solnica-Krezel laboratory for use of their confocal microscope and RhoA construct, and Marilyn Levy for assistance with transmission electron microscopy. Finally, we thank Charleen Johnson and the Washington University Zebrafish Consortium staff for excellent fish care. This work was supported by a predoctoral fellowship from the NIH to S.D.A. (NS087801), a predoctoral fellowship from the NIH to C.G. (NS079047), and by grants to K.R.M. from the NIH (R01 NS079445) and the Edward J. Mallinckrodt Foundation. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",
year = "2015",
month = jan,
day = "21",
doi = "10.1038/ncomms7122",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
}