@article{8b05fe5a355f464d84afa83f8f3be6c9,
title = "The absence of a microbiota enhances TSLP expression in mice with defective skin barrier but does not affect the severity of their allergic inflammation",
abstract = "Evidence is accumulating to suggest that our indigenous microbial communities (microbiota) may have a role in modulating allergic and immune disorders of the skin. To examine the link between the microbiota and atopic dermatitis (AD), we examined a mouse model of defective cutaneous barrier function with an AD-like disease due to loss of Notch signaling. Comparisons of conventionally raised and germ-free (GF) mice revealed a similar degree of allergic skin inflammation, systemic atopy, and airway hypersensitivity. GF mutant animals expressed significantly higher levels of thymic stromal lymphopoietin, a major proinflammatory cytokine released by skin with defective barrier function, resulting in a more severe B-lymphoproliferative disorder that persisted into adulthood. These findings suggest a role for the microbiota in ameliorating stress signals released by keratinocytes in response to perturbation in cutaneous barrier function.",
author = "Yockey, {Laura J.} and Shadmehr Demehri and Mustafa Turkoz and Ahu Turkoz and Ahern, {Philip P.} and Omar Jassim and Sindhu Manivasagam and Kearney, {John F.} and Gordon, {Jeffrey I.} and Raphael Kopan",
note = "Funding Information: We thank Michael Holtzman for helpful discussions and many valuable reagents, and the members of the Kopan laboratory for their suggestions during the course of this study. We also thank Gail Martin for providing Msx2-Cretg/+ mice, Tasuku Honjo for Rbpj flox/flox mice, and Dave O{\textquoteright}Donnell, Maria Karlsson, and other members of the Gordon lab for their assistance with and advice about gnotobiotic husbandry. This work was supported with funds from the American Asthma Foundation (grant 09-0234) to RK. AT was supported by NIH AI070489, JK by NIAID AI14782-33, and JIG by NIH grant P30DRO52574 (Murine Model Core of the Washington University Digestive Disease Research Core Center) and by the Crohn{\textquoteright}s and Colitis Association of America. ",
year = "2013",
month = dec,
doi = "10.1038/jid.2013.228",
language = "English",
volume = "133",
pages = "2714--2721",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
number = "12",
}