@article{ad65d1222b4b4d1a99aee4025ca81feb,
title = "The β3-integrin endothelial adhesome regulates microtubule-dependent cell migration",
abstract = "Integrin β3 is seen as a key anti-angiogenic target for cancer treatment due to its expression on neovasculature, but the role it plays in the process is complex; whether it is pro- or anti-angiogenic depends on the context in which it is expressed. To understand precisely β3's role in regulating integrin adhesion complexes in endothelial cells, we characterised, by mass spectrometry, the β3-dependent adhesome. We show that depletion of β3-integrin in this cell type leads to changes in microtubule behaviour that control cell migration. β3-integrin regulates microtubule stability in endothelial cells through Rcc2/Anxa2-driven control of active Rac1 localisation. Our findings reveal that angiogenic processes, both in vitro and in vivo, are more sensitive to microtubule targeting agents when β3-integrin levels are reduced.",
keywords = "adhesome, endothelial, integrins, microtubules",
author = "Atkinson, {Samuel J.} and Gontarczyk, {Aleksander M.} and Alghamdi, {Abdullah A.A.} and Ellison, {Tim S.} and Johnson, {Robert T.} and Fowler, {Wesley J.} and Kirkup, {Benjamin M.} and Silva, {Bernardo C.} and Harry, {Bronwen E.} and Schneider, {Jochen G.} and Weilbaecher, {Katherine N.} and Mogensen, {Mette M.} and Bass, {Mark D.} and Maddy Parsons and Edwards, {Dylan R.} and Robinson, {Stephen D.}",
note = "Funding Information: This work was part funded by: BBSRC DTP PhD studentships to SJA (BB/ J014524/1), RTJ (BB/M011216/1), WJF (BB/M011216/1) and BMK (BB/J014524/1); a BigC PhD studentship to AMG (14-06R); BBSRC funding to MMM (BB/J009040/1); Wellcome Trust funding to MDB (088419); Universit{\'e} du Luxembourg core funding to JGS (DFG SCH682/3-1, FNR Core ITGB3 VascIn); and BHF funding to SDR (PG/15/25/31369). The authors acknowledge special thanks to both Drs Sophie Akbareian and Peng Liu for their undying enthusiastic and critical support of this project. Additionally, we thank Norfolk Fundraisers, Mrs Margaret Doggett, and the Colin Wright Fund for their kind support and fundraising over the years. Publisher Copyright: {\textcopyright} 2018 The Authors. Published under the terms of the CC BY 4.0 license",
year = "2018",
month = jul,
doi = "10.15252/embr.201744578",
language = "English",
volume = "19",
journal = "EMBO Reports",
issn = "1469-221X",
number = "7",
}