The α5β1 fibronectin receptor: Characterization of the α5 gene promoter

T. M. Birkenmeier, J. J. McQuillan, E. D. Boedeker, W. S. Argraves, E. Ruoslahti, D. C. Dean

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A genomic clone containing the gene for the α5 subunit of the human α5β1 integrin complex was isolated by screening a human genomic library with the previously described α5 cDNA (Argraves, W. S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M. D., and Ruoslahti, E. (1987) J. Cell Biol 105, 1183-1190). The α5 gene 5'-flanking region lacks both TATA and CCAAT boxes, and it is located in a CpG island. This region was an active promoter in transfection assays using the HT-1080 cell line (fibrosarcoma), which expresses α5, but was inactive in the Raji cell line (B cell), which does not express α5. These results indicate that the α5 gene 5'-flanking region acts as a promoter that exhibits the expected cell-type specificity. Deletion of α5 promoter sequences from position -657 to -178 caused transcription stimulation, suggesting that a silencer is located between these sites. Successive 5' deletions from position -178 decreased promoter activity until activity was essentially eliminated on deletion to position -27. Isolation of a functional promoter for the α5 gene is a first step in understanding how expression of this gene is controlled at the molecular level.

Original languageEnglish
Pages (from-to)20544-20549
Number of pages6
JournalJournal of Biological Chemistry
Issue number30
StatePublished - 1991

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