TY - JOUR
T1 - The α5β1 fibronectin receptor
T2 - Characterization of the α5 gene promoter
AU - Birkenmeier, T. M.
AU - McQuillan, J. J.
AU - Boedeker, E. D.
AU - Argraves, W. S.
AU - Ruoslahti, E.
AU - Dean, D. C.
PY - 1991
Y1 - 1991
N2 - A genomic clone containing the gene for the α5 subunit of the human α5β1 integrin complex was isolated by screening a human genomic library with the previously described α5 cDNA (Argraves, W. S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M. D., and Ruoslahti, E. (1987) J. Cell Biol 105, 1183-1190). The α5 gene 5'-flanking region lacks both TATA and CCAAT boxes, and it is located in a CpG island. This region was an active promoter in transfection assays using the HT-1080 cell line (fibrosarcoma), which expresses α5, but was inactive in the Raji cell line (B cell), which does not express α5. These results indicate that the α5 gene 5'-flanking region acts as a promoter that exhibits the expected cell-type specificity. Deletion of α5 promoter sequences from position -657 to -178 caused transcription stimulation, suggesting that a silencer is located between these sites. Successive 5' deletions from position -178 decreased promoter activity until activity was essentially eliminated on deletion to position -27. Isolation of a functional promoter for the α5 gene is a first step in understanding how expression of this gene is controlled at the molecular level.
AB - A genomic clone containing the gene for the α5 subunit of the human α5β1 integrin complex was isolated by screening a human genomic library with the previously described α5 cDNA (Argraves, W. S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M. D., and Ruoslahti, E. (1987) J. Cell Biol 105, 1183-1190). The α5 gene 5'-flanking region lacks both TATA and CCAAT boxes, and it is located in a CpG island. This region was an active promoter in transfection assays using the HT-1080 cell line (fibrosarcoma), which expresses α5, but was inactive in the Raji cell line (B cell), which does not express α5. These results indicate that the α5 gene 5'-flanking region acts as a promoter that exhibits the expected cell-type specificity. Deletion of α5 promoter sequences from position -657 to -178 caused transcription stimulation, suggesting that a silencer is located between these sites. Successive 5' deletions from position -178 decreased promoter activity until activity was essentially eliminated on deletion to position -27. Isolation of a functional promoter for the α5 gene is a first step in understanding how expression of this gene is controlled at the molecular level.
UR - http://www.scopus.com/inward/record.url?scp=0025830171&partnerID=8YFLogxK
M3 - Article
C2 - 1834647
AN - SCOPUS:0025830171
VL - 266
SP - 20544
EP - 20549
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 30
ER -