TGF-β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue

Hongting Jin; Jie Shen; Baoli Wang; Meina Wang; Bing Shu; Di Chen

doi:10.1016/j.febslet.2011.03.034

TGF-β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue

Hongting Jin, Jie Shen, Baoli Wang, Meina Wang, Bing Shu, Di Chen

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Abstract

TGF-β signaling plays a critical role in cartilage and spine tissue development at embryonic stage but its role in postnatal intervertebral disc (IVD) tissue growth and maintenance remain poorly understood. In the present studies, we have deleted the Tgfbr2 gene in inner annulus fibrosus cells of the disc tissue and surrounding growth plate chondrocytes using Col2a1-CreER ^T2 transgenic mice. We found that TGF-β signaling is required for normal growth plate cartilage and endplate cartilage growth at postnatal stage. The expression of Mmp13 gene is significantly up-regulated in primary disc cells of Tgfbr2 conditional knockout mice. Deletion of the Mmp13 gene under Tgfbr2 null background completely reverses the abnormal disc phenotype found in Tgfbr2 knockout mice.

Original language	English
Pages (from-to)	1209-1215
Number of pages	7
Journal	FEBS Letters
Volume	585
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2011.03.034
State	Published - Apr 20 2011

Keywords

Col2a1-CreER
Conditional knockout
Intervertebral disc
MMP-13
TGF-β

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10.1016/j.febslet.2011.03.034

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title = "TGF-β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue",

abstract = "TGF-β signaling plays a critical role in cartilage and spine tissue development at embryonic stage but its role in postnatal intervertebral disc (IVD) tissue growth and maintenance remain poorly understood. In the present studies, we have deleted the Tgfbr2 gene in inner annulus fibrosus cells of the disc tissue and surrounding growth plate chondrocytes using Col2a1-CreER T2 transgenic mice. We found that TGF-β signaling is required for normal growth plate cartilage and endplate cartilage growth at postnatal stage. The expression of Mmp13 gene is significantly up-regulated in primary disc cells of Tgfbr2 conditional knockout mice. Deletion of the Mmp13 gene under Tgfbr2 null background completely reverses the abnormal disc phenotype found in Tgfbr2 knockout mice.",

keywords = "Col2a1-CreER, Conditional knockout, Intervertebral disc, MMP-13, TGF-β",

author = "Hongting Jin and Jie Shen and Baoli Wang and Meina Wang and Bing Shu and Di Chen",

note = "Funding Information: This work was supported by Grants R01 AR055915, R01 AR054465 and Contract C024320 to D.C. from National Institute of Health and New York State Department of Health and Empire State Stem Cell Board. This work was also partially supported by Grants Z2111261 and Y2111184 to D.C. and H.J. from Zhejiang Natural Science Foundation , China.",

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doi = "10.1016/j.febslet.2011.03.034",

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AU - Jin, Hongting

AU - Shen, Jie

AU - Wang, Baoli

AU - Wang, Meina

AU - Shu, Bing

AU - Chen, Di

N1 - Funding Information: This work was supported by Grants R01 AR055915, R01 AR054465 and Contract C024320 to D.C. from National Institute of Health and New York State Department of Health and Empire State Stem Cell Board. This work was also partially supported by Grants Z2111261 and Y2111184 to D.C. and H.J. from Zhejiang Natural Science Foundation , China.

PY - 2011/4/20

Y1 - 2011/4/20

N2 - TGF-β signaling plays a critical role in cartilage and spine tissue development at embryonic stage but its role in postnatal intervertebral disc (IVD) tissue growth and maintenance remain poorly understood. In the present studies, we have deleted the Tgfbr2 gene in inner annulus fibrosus cells of the disc tissue and surrounding growth plate chondrocytes using Col2a1-CreER T2 transgenic mice. We found that TGF-β signaling is required for normal growth plate cartilage and endplate cartilage growth at postnatal stage. The expression of Mmp13 gene is significantly up-regulated in primary disc cells of Tgfbr2 conditional knockout mice. Deletion of the Mmp13 gene under Tgfbr2 null background completely reverses the abnormal disc phenotype found in Tgfbr2 knockout mice.

AB - TGF-β signaling plays a critical role in cartilage and spine tissue development at embryonic stage but its role in postnatal intervertebral disc (IVD) tissue growth and maintenance remain poorly understood. In the present studies, we have deleted the Tgfbr2 gene in inner annulus fibrosus cells of the disc tissue and surrounding growth plate chondrocytes using Col2a1-CreER T2 transgenic mice. We found that TGF-β signaling is required for normal growth plate cartilage and endplate cartilage growth at postnatal stage. The expression of Mmp13 gene is significantly up-regulated in primary disc cells of Tgfbr2 conditional knockout mice. Deletion of the Mmp13 gene under Tgfbr2 null background completely reverses the abnormal disc phenotype found in Tgfbr2 knockout mice.

KW - Col2a1-CreER

KW - Conditional knockout

KW - Intervertebral disc

KW - MMP-13

KW - TGF-β

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SN - 0014-5793

VL - 585

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JO - FEBS Letters

JF - FEBS Letters

IS - 8

ER -

TGF-β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue

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Keywords

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