TY - JOUR
T1 - Texture analysis of poly-adenylated mRNA staining following global brain ischemia and reperfusion
AU - Szymanski, Jeffrey J.
AU - Jamison, Jill T.
AU - DeGracia, Donald J.
N1 - Funding Information:
We thank Kirsi Holli, Medical Imaging Centre at Tampere University Hospital, Finland, for her helpful advice on using MaZda. We thank Khan M. Iftekharuddin and Shaheen Ahmed at the Institute for Intelligent Systems, University of Memphis, for their kind help and advice on calculating the fractal dimension. We thank Jie Wang for her assistance with the 2VO/HT model. This work was supported by the National Institute of Neurological Disorders and Stroke , NS057167 (D.J.D.) and a Ruth L. Kirschstein National Research Service Award , NS063651 (J.J.S.), and a Thomas C. Rumble Fellowship, Wayne State University (J.T.J.).
PY - 2012/1
Y1 - 2012/1
N2 - Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1. h of reperfusion after 10. min of normothermic global cerebral ischemia. At 1. h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.
AB - Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1. h of reperfusion after 10. min of normothermic global cerebral ischemia. At 1. h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.
KW - Brain ischemia and reperfusion
KW - Cycloheximide
KW - Fractal dimension
KW - MRNA granules
KW - Texture analysis
UR - http://www.scopus.com/inward/record.url?scp=84855202774&partnerID=8YFLogxK
U2 - 10.1016/j.cmpb.2011.03.005
DO - 10.1016/j.cmpb.2011.03.005
M3 - Article
C2 - 21477879
AN - SCOPUS:84855202774
SN - 0169-2607
VL - 105
SP - 81
EP - 94
JO - Computer Methods and Programs in Biomedicine
JF - Computer Methods and Programs in Biomedicine
IS - 1
ER -