Texture analysis of poly-adenylated mRNA staining following global brain ischemia and reperfusion

Jeffrey J. Szymanski, Jill T. Jamison, Donald J. DeGracia

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1. h of reperfusion after 10. min of normothermic global cerebral ischemia. At 1. h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.

Original languageEnglish
Pages (from-to)81-94
Number of pages14
JournalComputer Methods and Programs in Biomedicine
Issue number1
StatePublished - Jan 2012


  • Brain ischemia and reperfusion
  • Cycloheximide
  • Fractal dimension
  • MRNA granules
  • Texture analysis


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