Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells

David S.J. Allan, Marco Colonna, Lewis L. Lanier, Tatyana D. Churakova, John S. Abrams, Shirley A. Ellis, Andrew J. McMichael, Veronique M. Braud

Research output: Contribution to journalArticlepeer-review

209 Scopus citations

Abstract

The nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal-fetal interface in pregnancy. It has long been suggested that HLA-G may inhibit maternal natural killer (NK) cells through interaction with particular NK cell receptors (KIRs). To investigate interactions of HLA-G, we constructed phycoerythrin-labeled tetrameric complexes of HLA-G refolded with a self-peptide. These HLA-G tetramers failed to bind to NK cells and cells transfected with CD94/NKG2 and killer immunoglobulin-like NK receptors. In contrast, HLA-G tetramers did bind to peripheral blood monocytes, staining a CD16+CD14(mid) subset with greater intensity. On transfectants, HLA-G tetramers bound to inhibitory immunoglobulin-like transcript (ILT)2 and ILT4 receptors. However, staining in the presence of antibodies reactive with ILT receptors revealed that the interaction of HLA-G tetramers with blood monocytes was largely due to binding to ILT4. These results suggest that the primary role of HLA-G may be the modulation of myelomonocytic cell behavior in pregnancy.

Original languageEnglish
Pages (from-to)1149-1155
Number of pages7
JournalJournal of Experimental Medicine
Volume189
Issue number7
DOIs
StatePublished - Apr 5 1999

Keywords

  • CD94
  • Immunoglobulin-like transcript
  • Killer immunoglobulin-like receptor
  • Monocyte
  • Natural killer cell

Fingerprint

Dive into the research topics of 'Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells'. Together they form a unique fingerprint.

Cite this