@article{bfc106fb9151410583f98c3b48789871,
title = "Temperature-sensitive male sterility in rice determined by the roles of AGO1d in reproductive phasiRNA biogenesis and function",
abstract = "Phased secondary siRNAs (phasiRNAs) are broadly present in the reproductive tissues of flowering plants, with spatial–temporal specificity. However, the ARGONAUTE (AGO) proteins associated with phasiRNAs and their miRNA triggers remain elusive. Here, through histological and high-throughput sequencing analyses, we show that rice AGO1d, which is specifically expressed in anther wall cells before and during meiosis, associates with both miR2118 and miR2275 to mediate phasiRNA biogenesis. AGO1d preferentially binds to miR2118-triggered 21-nucleotide (nt) phasiRNAs with a 5′-terminal uridine, suggesting a dual role in phasiRNA biogenesis and function. Depletion of AGO1d causes a reduction of 21- and 24-nt phasiRNAs and temperature-sensitive male sterility. At lower temperatures, anthers of the ago1d mutant predominantly show excessive tapetal cells with little starch accumulation during pollen formation, possibly caused by the dysregulation of cell metabolism. These results uncover an essential role of AGO1d in rice anther development at lower temperatures and demonstrate coordinative roles of AGO proteins during reproductive phasiRNA biogenesis and function.",
keywords = "Argonaute, anther, microRNA, phasiRNA, rice",
author = "Chuanlin Shi and Jie Zhang and Bingjin Wu and Rachel Jouni and Changxiu Yu and Meyers, {Blake C.} and Wanqi Liang and Qili Fei",
note = "Funding Information: We thank members from the Fei Lab, Liang Lab, and Meyers Lab for helpful discussions. We thank Joanna Friesner for assistance in editing the text. This study was supported by the Guangdong Basic and Applied Basic Research Foundation (2019A1515110868), National Natural Science Foundation of China (32100289, U19A2031), and special funds for science technology innovation and industrial development of Shenzhen Dapeng New District (KJYF202001‐01) to QF, and a US National Science Foundation award (no. 1754097) to BCM. Funding Information: We thank members from the Fei Lab, Liang Lab, and Meyers Lab for helpful discussions. We thank Joanna Friesner for assistance in editing the text. This study was supported by the Guangdong Basic and Applied Basic Research Foundation (2019A1515110868), National Natural Science Foundation of China (32100289, U19A2031), and special funds for science technology innovation and industrial development of Shenzhen Dapeng New District (KJYF202001-01) to QF, and a US National Science Foundation award (no. 1754097) to BCM. Publisher Copyright: {\textcopyright} 2022 The Authors. New Phytologist {\textcopyright} 2022 New Phytologist Foundation.",
year = "2022",
month = nov,
doi = "10.1111/nph.18446",
language = "English",
volume = "236",
pages = "1529--1544",
journal = "New Phytologist",
issn = "0028-646X",
number = "4",
}