Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats

Nada A. Abumrad; Patty Briscoe; Albert H. Beth; Richard R. Whitesell

doi:10.1016/0005-2736(88)90161-7

Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats

Nada A. Abumrad, Patty Briscoe, Albert H. Beth, Richard R. Whitesell

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below 30°C and decreased them above 30°C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at 14°C by increasing V_max. The effect was slight at 40°C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at 40°C as a result of a rise in the K_m, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport K_m at physiological temperatures only.

Original language	English
Pages (from-to)	222-230
Number of pages	9
Journal	BBA - Biomembranes
Volume	938
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(88)90161-7
State	Published - Feb 18 1988

Keywords

(Rat)
Alloxan diabetes
Erythrocyte
Glucose transport
Temperature dependence

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10.1016/0005-2736(88)90161-7

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title = "Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats",

abstract = "Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below 30°C and decreased them above 30°C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at 14°C by increasing Vmax. The effect was slight at 40°C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at 40°C as a result of a rise in the Km, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport Km at physiological temperatures only.",

keywords = "(Rat), Alloxan diabetes, Erythrocyte, Glucose transport, Temperature dependence",

author = "Abumrad, {Nada A.} and Patty Briscoe and Beth, {Albert H.} and Whitesell, {Richard R.}",

note = "Funding Information: The authors are grateful to Drs. Charles R. Park and David M. Regen for many helpful discussions and to Patricia Perry and Saul Juliao for their efficient technical assistance. This work was supported by an American Diabetes Association grant, by a grant from the Juvenile Diabetes Foundation 82R604 and by NIH grant R01 HL34737.",

year = "1988",

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doi = "10.1016/0005-2736(88)90161-7",

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T1 - Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats

AU - Abumrad, Nada A.

AU - Briscoe, Patty

AU - Beth, Albert H.

AU - Whitesell, Richard R.

N1 - Funding Information: The authors are grateful to Drs. Charles R. Park and David M. Regen for many helpful discussions and to Patricia Perry and Saul Juliao for their efficient technical assistance. This work was supported by an American Diabetes Association grant, by a grant from the Juvenile Diabetes Foundation 82R604 and by NIH grant R01 HL34737.

PY - 1988/2/18

Y1 - 1988/2/18

N2 - Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below 30°C and decreased them above 30°C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at 14°C by increasing Vmax. The effect was slight at 40°C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at 40°C as a result of a rise in the Km, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport Km at physiological temperatures only.

AB - Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below 30°C and decreased them above 30°C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at 14°C by increasing Vmax. The effect was slight at 40°C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at 40°C as a result of a rise in the Km, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport Km at physiological temperatures only.

KW - (Rat)

KW - Alloxan diabetes

KW - Erythrocyte

KW - Glucose transport

KW - Temperature dependence

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Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats

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