Temperature dependence of glucose transport in erythrocytes from normal and alloxan-diabetic rats

Nada A. Abumrad, Patty Briscoe, Albert H. Beth, Richard R. Whitesell

Research output: Contribution to journalArticle

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Abstract

Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below 30°C and decreased them above 30°C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at 14°C by increasing Vmax. The effect was slight at 40°C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at 40°C as a result of a rise in the Km, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport Km at physiological temperatures only.

Original languageEnglish
Pages (from-to)222-230
Number of pages9
JournalBBA - Biomembranes
Volume938
Issue number2
DOIs
StatePublished - Feb 18 1988

Keywords

  • (Rat)
  • Alloxan diabetes
  • Erythrocyte
  • Glucose transport
  • Temperature dependence

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