Technetium 99m labeling of human monocytes for chemotactic studies

C. K. Papierniak, R. E. Bourey, R. R. Kretschmer, S. P. Gotoff, L. G. Colombetti

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium 99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte derived chemotactic factor. The chemotactic properties of 99mTc labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.

Original languageEnglish
Pages (from-to)988-992
Number of pages5
JournalJournal of Nuclear Medicine
Volume17
Issue number11
StatePublished - 1976

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