Technetium 99m labeling of human monocytes for chemotactic studies

C. K. Papierniak; R. E. Bourey; R. R. Kretschmer; S. P. Gotoff; L. G. Colombetti

Technetium 99m labeling of human monocytes for chemotactic studies

C. K. Papierniak, R. E. Bourey, R. R. Kretschmer, S. P. Gotoff, L. G. Colombetti

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Abstract

Although the current use of ⁵¹Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium 99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte derived chemotactic factor. The chemotactic properties of ⁹⁹mTc labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.

Original language	English
Pages (from-to)	988-992
Number of pages	5
Journal	Journal of Nuclear Medicine
Volume	17
Issue number	11
State	Published - 1976

Cite this

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title = "Technetium 99m labeling of human monocytes for chemotactic studies",

abstract = "Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium 99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte derived chemotactic factor. The chemotactic properties of 99mTc labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.",

author = "Papierniak, {C. K.} and Bourey, {R. E.} and Kretschmer, {R. R.} and Gotoff, {S. P.} and Colombetti, {L. G.}",

year = "1976",

language = "English",

volume = "17",

pages = "988--992",

journal = "Journal of Nuclear Medicine",

issn = "0161-5505",

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T1 - Technetium 99m labeling of human monocytes for chemotactic studies

AU - Papierniak, C. K.

AU - Bourey, R. E.

AU - Kretschmer, R. R.

AU - Gotoff, S. P.

AU - Colombetti, L. G.

PY - 1976

Y1 - 1976

N2 - Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium 99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte derived chemotactic factor. The chemotactic properties of 99mTc labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.

AB - Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium 99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte derived chemotactic factor. The chemotactic properties of 99mTc labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.

UR - http://www.scopus.com/inward/record.url?scp=0017202878&partnerID=8YFLogxK

M3 - Article

C2 - 978262

AN - SCOPUS:0017202878

SN - 0161-5505

VL - 17

SP - 988

EP - 992

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

IS - 11

ER -

Technetium 99m labeling of human monocytes for chemotactic studies

Abstract

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