@article{e974176130af4b3993d4f239c084d835,
title = "Targeted PET imaging of chemokine receptor 2-positive monocytes and macrophages in the injured heart",
abstract = "Proinflammatory macrophages are important mediators of inflammation after myocardial infarction and of allograft injury after heart transplantation. The aim of this study was to image the recruitment of proinflammatory chemokine receptor 2-positive (CCR21) cells in multiple heart injury models. Methods: 64Cu-DOTA-extracellular loop 1 inverso (ECL1i) PET was used to image CCR21 monocytes and macrophages in a heart transplantation mouse model. Flow cytometry was performed to characterize CCR21 cells. Autoradiography on a human heart specimen was conducted to confirm binding specificity. 64Cu- and 68Ga-DOTA-ECL1i were compared in an ischemia-reperfusion injury mouse model. Results: 64Cu-DOTA-ECL1i showed sensitive and specific detection of CCR21 cells in all tested mouse models, with efficacy comparable to that of 68Ga-DOTA-ECL1i. Flow cytometry demonstrated specific expression of CCR2 on monocytes and macrophages. The tracer binds to human CCR2. Conclusion: This work establishes the utility of 64Cu-DOTA-ECL1i to image CCR21 monocytes and macrophages in mouse models and provides the requisite preclinical information to translate the targeted clinical-grade CCR2 imaging probe for clinical investigation of heart diseases.",
keywords = "C-C chemokine receptor type 2 (CCR2), Heart injury, Macrophages, Monocytes, PET",
author = "Heo, {Gyu Seong} and Geetika Bajpai and Wenjun Li and Luehmann, {Hannah P.} and Sultan, {Deborah H.} and Hao Dun and Florian Leuschner and Brody, {Steven L.} and Gropler, {Robert J.} and Daniel Kreisel and Lavine, {Kory J.} and Yongjian Liu",
note = "Funding Information: Yongjian Liu, Daniel Kreisel, Robert Gropler, Steven Brody, and Kory Lavine have a pending patent entitled {\textquoteleft}{\textquoteleft}Compositions and Methods for Detecting CCR2 Receptors{\textquoteright}{\textquoteright} (application 15/ 611,577). Daniel Kreisel serves on the Scientific Advisory Board of Compass Therapeutics. Yongjian Liu is supported by NIH R35 HL145212, R01 HL131908, and P41 EB025815. Kory Lavine is supported by NIH K08 HL123519, R01 HL138466, R01 HL139714, the Burroughs Welcome Fund (1014782), the Children{\textquoteright}s Discovery Institute of Washington University and St. Louis Children{\textquoteright}s Hospital (CHII-2015–462 and CH-II-2017–628), and the Foundation of Barnes–Jewish Hospital (8038-88). Daniel Kreisel is supported by 1P01AI116501, R01 HL094601, Veterans Administration Merit Review grant 1I01BX002730, and the Foundation for Barnes–Jewish Hospital. Steven Brody is supported by R01 HL131908 and is the Dorothy R. and Hubert C. Moog Pro-fessor of Pulmonary Medicine, awarded through the Barnes–Jewish Hospital Founda-tion. Robert Gropler is supported by P41 EB025815. No other potential conflict of in-terest relevant to this article was reported. Publisher Copyright: COPYRIGHT {\textcopyright} 2021 by the Society of Nuclear Medicine and Molecular Imaging.",
year = "2021",
month = jan,
day = "1",
doi = "10.2967/jnumed.120.244673",
language = "English",
volume = "62",
pages = "111--114",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
number = "1",
}