Targeted PARACEST nanoparticle contrast agent for the detection of fibrin

Patrick M. Winter; Kejia Cai; Junjie Chen; Christopher R. Adair; Garry E. Kiefer; Phillip S. Athey; Patrick J. Gaffney; Carolyn E. Buff; J. David Robertson; Shelton D. Caruthers; Samuel A. Wickline; Gregory M. Lanza

doi:10.1002/mrm.21093

Targeted PARACEST nanoparticle contrast agent for the detection of fibrin

Patrick M. Winter, Kejia Cai, Junjie Chen, Christopher R. Adair, Garry E. Kiefer, Phillip S. Athey, Patrick J. Gaffney, Carolyn E. Buff, J. David Robertson, Shelton D. Caruthers, Samuel A. Wickline, Gregory M. Lanza

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

A lipid-encapsulated perfluorocarbon nanoparticle molecular imaging contrast agent that utilizes a paramagnetic chemical exchange saturation transfer (PARACEST) chelate is presented. PARACEST agents are ideally suited for molecular imaging applications because one can switch the contrast on and off at will simply by adjusting the pulse sequence parameters. This obviates the need for pre- and postinjection images to define contrast agent binding. Spectroscopy (4.7T) of PARACEST nanoparticles revealed a bound water peak at 52 ppm, in agreement with results from the water-soluble chelate. Imaging of control nanoparticles showed no appreciable contrast, while PARACEST nanoparticles produced >10% signal enhancement. PARACEST nanoparticles were targeted to clots via antifibrin antibodies and produced a contrast-to-noise ratio (CNR) of 10 at the clot surface.

Original language	English
Pages (from-to)	1384-1388
Number of pages	5
Journal	Magnetic resonance in medicine
Volume	56
Issue number	6
DOIs	https://doi.org/10.1002/mrm.21093
State	Published - Dec 2006

Keywords

Contrast agent
Fibrin
MRI
Molecular imaging
Nanoparticle

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10.1002/mrm.21093

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title = "Targeted PARACEST nanoparticle contrast agent for the detection of fibrin",

abstract = "A lipid-encapsulated perfluorocarbon nanoparticle molecular imaging contrast agent that utilizes a paramagnetic chemical exchange saturation transfer (PARACEST) chelate is presented. PARACEST agents are ideally suited for molecular imaging applications because one can switch the contrast on and off at will simply by adjusting the pulse sequence parameters. This obviates the need for pre- and postinjection images to define contrast agent binding. Spectroscopy (4.7T) of PARACEST nanoparticles revealed a bound water peak at 52 ppm, in agreement with results from the water-soluble chelate. Imaging of control nanoparticles showed no appreciable contrast, while PARACEST nanoparticles produced >10% signal enhancement. PARACEST nanoparticles were targeted to clots via antifibrin antibodies and produced a contrast-to-noise ratio (CNR) of 10 at the clot surface.",

keywords = "Contrast agent, Fibrin, MRI, Molecular imaging, Nanoparticle",

author = "Winter, {Patrick M.} and Kejia Cai and Junjie Chen and Adair, {Christopher R.} and Kiefer, {Garry E.} and Athey, {Phillip S.} and Gaffney, {Patrick J.} and Buff, {Carolyn E.} and Robertson, {J. David} and Caruthers, {Shelton D.} and Wickline, {Samuel A.} and Lanza, {Gregory M.}",

year = "2006",

month = dec,

doi = "10.1002/mrm.21093",

language = "English",

volume = "56",

pages = "1384--1388",

journal = "Magnetic resonance in medicine",

issn = "0740-3194",

number = "6",

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T1 - Targeted PARACEST nanoparticle contrast agent for the detection of fibrin

AU - Winter, Patrick M.

AU - Cai, Kejia

AU - Chen, Junjie

AU - Adair, Christopher R.

AU - Kiefer, Garry E.

AU - Athey, Phillip S.

AU - Gaffney, Patrick J.

AU - Buff, Carolyn E.

AU - Robertson, J. David

AU - Caruthers, Shelton D.

AU - Wickline, Samuel A.

AU - Lanza, Gregory M.

PY - 2006/12

Y1 - 2006/12

N2 - A lipid-encapsulated perfluorocarbon nanoparticle molecular imaging contrast agent that utilizes a paramagnetic chemical exchange saturation transfer (PARACEST) chelate is presented. PARACEST agents are ideally suited for molecular imaging applications because one can switch the contrast on and off at will simply by adjusting the pulse sequence parameters. This obviates the need for pre- and postinjection images to define contrast agent binding. Spectroscopy (4.7T) of PARACEST nanoparticles revealed a bound water peak at 52 ppm, in agreement with results from the water-soluble chelate. Imaging of control nanoparticles showed no appreciable contrast, while PARACEST nanoparticles produced >10% signal enhancement. PARACEST nanoparticles were targeted to clots via antifibrin antibodies and produced a contrast-to-noise ratio (CNR) of 10 at the clot surface.

AB - A lipid-encapsulated perfluorocarbon nanoparticle molecular imaging contrast agent that utilizes a paramagnetic chemical exchange saturation transfer (PARACEST) chelate is presented. PARACEST agents are ideally suited for molecular imaging applications because one can switch the contrast on and off at will simply by adjusting the pulse sequence parameters. This obviates the need for pre- and postinjection images to define contrast agent binding. Spectroscopy (4.7T) of PARACEST nanoparticles revealed a bound water peak at 52 ppm, in agreement with results from the water-soluble chelate. Imaging of control nanoparticles showed no appreciable contrast, while PARACEST nanoparticles produced >10% signal enhancement. PARACEST nanoparticles were targeted to clots via antifibrin antibodies and produced a contrast-to-noise ratio (CNR) of 10 at the clot surface.

KW - Contrast agent

KW - Fibrin

KW - MRI

KW - Molecular imaging

KW - Nanoparticle

UR - http://www.scopus.com/inward/record.url?scp=33845259382&partnerID=8YFLogxK

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DO - 10.1002/mrm.21093

M3 - Article

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AN - SCOPUS:33845259382

SN - 0740-3194

VL - 56

SP - 1384

EP - 1388

JO - Magnetic resonance in medicine

JF - Magnetic resonance in medicine

IS - 6

ER -

Targeted PARACEST nanoparticle contrast agent for the detection of fibrin

Abstract

Keywords

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