Targeted loss of proteoglycans results in changes of frequency-dependent viscoelastic behavior of the intact articular cartilage

Simon Y. Tang, Tamara Alliston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cartilage is a multi-phasic, viscoelastic material that derives its mechanical behavior of its primary constituents including collagen, proteoglycans, and water. The complex mechanical function of cartilage depends critically on the composition and balance of these constituents. We sought to determine the effects of proteoglycan loss on both the time- and frequency-dependent mechanical behavior of articular cartilage. Using cathepsin d, an enzyme that specifically cleaves proteoglycans, we assessed the in situ mechanical behavior of intact bovine articular cartilage before and after enzymatic digestion using microindentation over loading frequencies ranging between 0.5 hz to 20 hz. The loss of proteoglycans does not affect the elastic components of mechanical behavior (indentation modulus; p=0.67), but have significant consequences on the viscoelastic components (tan δ; p<0.001). Moreover, the changes in the viscoelastic mechanical behavior are more pronounced at higher loading frequencies (p<0.001). Taken together, these results suggest that proteoglycans are critical for providing dynamic stability for the cartilage tissue.

Original languageEnglish
Title of host publicationASME 2012 Summer Bioengineering Conference, SBC 2012
Pages299-300
Number of pages2
DOIs
StatePublished - Dec 1 2012
Externally publishedYes
EventASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico
Duration: Jun 20 2012Jun 23 2012

Publication series

NameASME 2012 Summer Bioengineering Conference, SBC 2012

Conference

ConferenceASME 2012 Summer Bioengineering Conference, SBC 2012
CountryPuerto Rico
CityFajardo
Period06/20/1206/23/12

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  • Cite this

    Tang, S. Y., & Alliston, T. (2012). Targeted loss of proteoglycans results in changes of frequency-dependent viscoelastic behavior of the intact articular cartilage. In ASME 2012 Summer Bioengineering Conference, SBC 2012 (pp. 299-300). (ASME 2012 Summer Bioengineering Conference, SBC 2012). https://doi.org/10.1115/SBC2012-80869