Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Zhongtao Hu; Yaoheng Yang; Dezhuang Ye; Si Chen; Yan Gong; Chinwendu Chukwu; Hong Chen

doi:10.1016/j.xpro.2023.102132

Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Zhongtao Hu, Yaoheng Yang, Dezhuang Ye, Si Chen, Yan Gong, Chinwendu Chukwu, Hong Chen

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).¹

Original language	English
Article number	102132
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2023.102132
State	Published - Mar 17 2023

Keywords

Biotechnology and bioengineering
Cancer
Health Sciences
Neuroscience
Physics

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10.1016/j.xpro.2023.102132

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title = "Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device",

abstract = "Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1",

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AU - Hu, Zhongtao

AU - Yang, Yaoheng

AU - Ye, Dezhuang

AU - Chen, Si

AU - Gong, Yan

AU - Chukwu, Chinwendu

AU - Chen, Hong

PY - 2023/3/17

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AB - Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1

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KW - Cancer

KW - Health Sciences

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Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

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