47 Scopus citations

Abstract

apobec-1 complementation factor (ACF) is an hnRNP family member which functions as the obligate RNA binding subunit of the core enzyme mediating C-to-U editing of the nuclear apolipoprotein B (apoB) transcript. ACF binds to both apoB RNA and apobec-1, the catalytic cytidine deaminase, which then results in site-specific posttranscriptional editing of apoB mRNA. Targeted deletion of apobec1 eliminates C-to-U editing of apoB mRNA but is otherwise well tolerated. However, the functions and potential targets of ACF beyond apoB mRNA editing are unknown. Here we report the results of generating acf knockout mice using homologous recombination. While heterozygous acf-/- mice were apparently healthy and fertile, no viable acf-/- mice were identified. Mutant acf-/- embryos were detectable only until the blastocyst (embryonic day 3.5 [E3.5]) stage. No acf-/- blastocysts were detectable following implantation at E4.5, and isolated acf-/- blastocysts failed to proliferate in vitro. Small interfering RNA knockdown of ACF in either rat (apobec-1-expressing) or human (apobec-1-deficient) hepatoma cells decreased ACF protein expression and induced a commensurate increase in apoptosis. Taken together, these data suggest that ACF plays a crucial role, which is independent of apobec-1 expression, in cell survival, particularly during early embryonic development.

Original languageEnglish
Pages (from-to)7260-7269
Number of pages10
JournalMolecular and cellular biology
Volume25
Issue number16
DOIs
StatePublished - Aug 2005

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