Target sequences for mutagenesis in Salmonella histidine‐requiring mutants

Philip E. Hartman, Bruce N. Ames, John R. Roth, Wayne M. Barnes, David E. Levin

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Nucleotide target sequences involved in reversion to the wild type phenotype are diagrammed for Salmonella frameshift histidine‐requiring mutants hisD3052, hisD3018, hisD6610, and hisD6580 and for base‐substitution mutants hisG46 and hisG428. Frameshift strain hisC3076 probably reverts by nucleotide changes similar to those that occur during reversion of hisD3018 and hisD6610. Multiple modes of reversion characterize each strain. Each strain also has a particularly diagnostic mutagen‐susceptible sequence. These highly mutagen‐susceptible stretches are the hisD3052 GCGCGCGC sequence, the hisD6610 CCCCCC sequence, the hisD6580 AAAAA sequence, and the A/T containing codon of hisG428 and G/C containing codon of hisG46, respectively. Between them, hisG46 and hisG428 are reverted by all of the six possible base substitution transition and transversion mutations.

Original languageEnglish
Pages (from-to)631-641
Number of pages11
JournalEnvironmental Mutagenesis
Volume8
Issue number4
DOIs
StatePublished - 1986

Keywords

  • Ames strains
  • bacteria
  • nucleotide sequences
  • reversions
  • review
  • suppressors

Fingerprint Dive into the research topics of 'Target sequences for mutagenesis in Salmonella histidine‐requiring mutants'. Together they form a unique fingerprint.

  • Cite this