TY - JOUR
T1 - Target selection for structural genomics of infectious diseases
AU - Yeats, Corin
AU - Dessailly, Benoit H.
AU - Glass, Elizabeth M.
AU - Fremont, Daved H.
AU - Orengo, Christine A.
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
PY - 2014
Y1 - 2014
N2 - This chapter describes the protocols used to identify, filter, and annotate potential protein targets from an organism associated with infectious diseases. Protocols often combine computational approaches for mining information in public databases or for checking whether the protein has already been targeted for structure determination, with manual strategies that examine the literature for information on the biological role of the protein or the experimental strategies that explore the effects of knocking out the protein. Publicly available computational tools have been cited as much as possible. Where these do not exist, the concepts underlying in-house tools developed for the Center for Structural Genomics of Infectious Diseases have been described.
AB - This chapter describes the protocols used to identify, filter, and annotate potential protein targets from an organism associated with infectious diseases. Protocols often combine computational approaches for mining information in public databases or for checking whether the protein has already been targeted for structure determination, with manual strategies that examine the literature for information on the biological role of the protein or the experimental strategies that explore the effects of knocking out the protein. Publicly available computational tools have been cited as much as possible. Where these do not exist, the concepts underlying in-house tools developed for the Center for Structural Genomics of Infectious Diseases have been described.
KW - Construct boundary identification
KW - Domain annotation
KW - Drug targets
KW - Essential proteins
KW - Secondary structure and disorder prediction
KW - Structure homologue recognition
KW - Target selection
KW - Vaccine and virulence targets
UR - http://www.scopus.com/inward/record.url?scp=84907942698&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0354-2_3
DO - 10.1007/978-1-4939-0354-2_3
M3 - Article
C2 - 24590707
AN - SCOPUS:84907942698
SN - 1064-3745
VL - 1140
SP - 35
EP - 51
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -