Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Methods: Col2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.
- Articular chondrocyte