Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice

M. Zhu, M. Chen, A. C. Lichtler, R. J. O'Keefe, D. Chen

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Methods: Col2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.

Original languageEnglish
Pages (from-to)129-130
Number of pages2
JournalOsteoarthritis and Cartilage
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Keywords

  • Articular chondrocyte
  • Cre-recombination
  • Tamoxifen

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