Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice

M. Zhu; M. Chen; A. C. Lichtler; R. J. O'Keefe; D. Chen

doi:10.1016/j.joca.2007.08.001

Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreER^T2 transgenic mice

M. Zhu, M. Chen, A. C. Lichtler, R. J. O'Keefe, D. Chen

Research output: Contribution to journal › Article › peer-review

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Abstract

Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER^T2 transgenic mice. Methods: Col2a1-CreER^T2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER^T2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreER^T2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.

Original language	English
Pages (from-to)	129-130
Number of pages	2
Journal	Osteoarthritis and Cartilage
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/j.joca.2007.08.001
State	Published - Jan 2008

Keywords

Articular chondrocyte
Cre-recombination
Tamoxifen

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10.1016/j.joca.2007.08.001

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title = "Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice",

abstract = "Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Methods: Col2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.",

keywords = "Articular chondrocyte, Cre-recombination, Tamoxifen",

author = "M. Zhu and M. Chen and Lichtler, {A. C.} and O'Keefe, {R. J.} and D. Chen",

note = "Funding Information: This work was supported by grants R01 AR051189, R01 AR054465 and K02 AR052411 to Di Chen from the National Institute of Health. ",

year = "2008",

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doi = "10.1016/j.joca.2007.08.001",

language = "English",

volume = "16",

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journal = "Osteoarthritis and Cartilage",

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T1 - Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice

AU - Zhu, M.

AU - Chen, M.

AU - Lichtler, A. C.

AU - O'Keefe, R. J.

AU - Chen, D.

N1 - Funding Information: This work was supported by grants R01 AR051189, R01 AR054465 and K02 AR052411 to Di Chen from the National Institute of Health.

PY - 2008/1

Y1 - 2008/1

N2 - Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Methods: Col2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.

AB - Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice. Methods: Col2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.

KW - Articular chondrocyte

KW - Cre-recombination

KW - Tamoxifen

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DO - 10.1016/j.joca.2007.08.001

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SN - 1063-4584

VL - 16

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JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

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ER -

Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreER^T2 transgenic mice

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Keywords

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