Tabtoxinine-β-lactam is a "stealth" β-lactam antibiotic that evades β-lactamase-mediated antibiotic resistance

Kathryn M. Hart, Margaret Reck, Gregory R. Bowman, Timothy A. Wencewicz

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Tabtoxinine-β-lactam (TβL) is a phytotoxin produced by plant pathogenic strains of Pseudomonas syringae. Unlike the majority of β-lactam antibiotics, TβL does not inhibit transpeptidase enzymes but instead is a potent, time-dependent inactivator of glutamine synthetase, an attractive and underexploited antibiotic target. TβL is produced by P. syringae in the form of a threonine dipeptide prodrug, tabtoxin (TβL-Thr), which enters plant and bacterial cells through dipeptide permeases. The role of β-lactamases in the self-protection of P. syringae from tabtoxin has been proposed, since this organism produces at least three β-lactamases. However, using in vitro and cellular assays and computational docking we have shown that TβL and TβL-Thr evade the action of all major classes of β-lactamase enzymes, thus overcoming the primary mechanism of resistance observed for traditional β-lactam antibiotics. TβL is a "stealth" β-lactam antibiotic and dipeptide prodrugs such as tabtoxin from P. syringae represent a novel antibiotic therapeutic strategy for treating multi-drug resistant Gram-negative pathogens expressing high levels of β-lactamase enzymes.

Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalMedChemComm
Volume7
Issue number1
DOIs
StatePublished - 2016

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