T-cell trafficking competence is required for CNS invasion

Jason R. Lees, Angela S. Archambault, John H. Russell

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

T-cell invasion of the CNS is critical for the induction of a variety of autoimmune mediated neuronal diseases. We utilized blood-brain barrier (BBB) mediated exclusion of anti-CD4 antibody to define populations of encephalitogenic T-cells recovered from mouse CNS preparations as either CNS invasive or non-invasive. This separation of cells allowed flow cytometric examination of the kinetics of encephalitogenic T-cell entry past the BBB. Further experiments examined the relative contribution of EAE inflammatory conditioning of the BBB to the kinetics of T-cell adherence and migration into the CNS. Inflammatory conditioning was found to have no effect on accumulation of T-cells at the vascular interface of the BBB, but was found to increase the entry of adoptively transferred T-cells into the CNS following their initial adherence to the BBB.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Neuroimmunology
Volume177
Issue number1-2
DOIs
StatePublished - Aug 1 2006

Keywords

  • Cell trafficking
  • EAE/MS
  • T-cells

Fingerprint Dive into the research topics of 'T-cell trafficking competence is required for CNS invasion'. Together they form a unique fingerprint.

  • Cite this