T cell recognition of bovine ribonuclease. Self/non-self discrimination at the level of binding to the I-A(k) molecule

R. G. Lorenz, A. N. Tyler, P. M. Allen

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Bovine RNase A specific T-cell hybridomas were generated to study the recognition of foreign Ag by T lymphocytes. One hybrid, TS12, was shown to recognize RNase in association with I-A(k). This hybridoma required bovine RNase to be processed before recognition. The immunogenic determinant on the RNase molecule recognized by TS12 was localized to the tryptic fragment RNase(40-61). All of the stimulatory ability of this determinant was shown to be contained within the synthetic 14mer RNase(43-56). When this segment of bovine RNase was compared with the self murine sequence, only one amino acid difference was found, a substitution of a proline residue at position 50 for a serine residue. This substitution completely abolishes binding to the I-A(k) molecule, as shown by both functional and direct binding assays. This finding shows that self/non-self discrimination not only occurs at the level of the T cell, but also can be caused by an inability of the self peptide to associate with a class II molecule.

Original languageEnglish
Pages (from-to)4124-4128
Number of pages5
JournalJournal of Immunology
Volume141
Issue number12
StatePublished - 1988

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