T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments.

H. S. Chou, M. A. Behlke, S. A. Godambe, J. H. Russell, C. G. Brooks, D. Y. Loh

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Both cDNA and genomic clones of the T cell receptor (TCR) alpha- and beta-chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non-functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six-member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7-14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general.

Original languageEnglish
Pages (from-to)2149-2155
Number of pages7
JournalThe EMBO journal
Volume5
Issue number9
StatePublished - Sep 1 1986
Externally publishedYes

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