T γ/δ hepatosplenic lymphoma in a heart transplant patient after an Epstein-Barr virus positive lymphoproliferative disorder: A case report

Madeleine D. Kraus, David F. Crawford, Zahid Kaleem, Shalini Shenoy, Craig A. MacArthur, Janina A. Longtine

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


BACKGROUND. An unusual case of a peripheral T-cell lymphoma of T γ/δ hepatosplenic type (Tγ/δHSL) that arose in a child 5 years after she received a heart transplant and 9 months after she developed Epstein-Barr virus (EBV) positive, B-cell lymphoid hyperplasia involving the tonsils is presented. The majority of the reported cases of Tγ/δHSL have been described in young adult men without antecedent immunodeficiency; several well documented cases of Tγ/δHSL in the posttransplant setting have been described previously, but none has been described in a child (or an adult) with a previously diagnosed EBV+ B-cell lymphoid hyperplasia. METHODS. Standard histologic, immunohistochemical, flow cytometric, and molecular genetic techniques were used in the evaluation of diagnostic material. RESULTS. The patient's Tγ/δHSL involved the spleen in a predominantly cordal pattern, and infiltrated the liver in an exclusively sinusoidal distribution. Bone marrow involvement was focal and interstitial. In all locations, malignant cells were of intermediate or large size and had oval nuclei with coarse chromatin, with a scant or moderate amount of eosinophilic cytoplasm. This Tγ/δHSL expressed the characteristic CD2+, CD3+, [CD4- CDR- ], Tδ1 + phenotype, and malignant cells also expressed the natural killer cell marker CD56. Cytogenetic studies demonstrated isochromosome 7q with the addition of trisomy 8 as the tumor progressed. Southern blot analysis demonstrated clonal rearrangements of the γ, δ, and β loci of the T-cell receptor but did not identify EBV DNA within the tumor cells. CONCLUSIONS. This case highlights the fact that a full range of lymphoid proliferations is possible in the posttransplantation period, and that a prior diagnosis of a B-cell disorder does not preclude the development of a subsequent T-cell posttransplant lymphoproliferative disorder (PTLD), which should be formally evaluated, especially if clinical circumstances appear atypical for a PTLD of the 'usual' (EBV-related, B-cell) type.

Original languageEnglish
Pages (from-to)983-992
Number of pages10
Issue number5
StatePublished - Mar 1 1998


  • Epstein-Barr virus
  • Lymphoma
  • Spleen
  • T-cell
  • Transplantation
  • γ/δ


Dive into the research topics of 'T γ/δ hepatosplenic lymphoma in a heart transplant patient after an Epstein-Barr virus positive lymphoproliferative disorder: A case report'. Together they form a unique fingerprint.

Cite this