TY - JOUR
T1 - Systematic analysis of the transcriptional switch inducing migration of border cells
AU - Borghese, Lodovica
AU - Fletcher, Georgina
AU - Mathieu, Juliette
AU - Atzberger, Ann
AU - Eades, William C.
AU - Cagan, Ross L.
AU - Rørth, Pernille
N1 - Funding Information:
We are very grateful to Silvie Ozon, Stefan Baumgartner, John Fessler, and Maria Leptin for antibodies; Lynn Cooley for both antibody and GFP-trap flies; and Andrew Jarman, Sandy Bernstein, John C. Sparrow, and the Bloomington stock center for mutants. We thank Jeff Gordon's lab and Mark A. Watson (Washington University) and Klaus-Michael Kuerner for technical advice and discussions; Ann Marie Voie for embryo injection; the Cagan and Rørth labs for help and discussions; and Eileen Furlong and Stephen Cohen for comments on the manuscript. G.F. was supported by a fellowship from DAAD. J.M. was supported by a fellowship from HFSP.
PY - 2006/4
Y1 - 2006/4
N2 - Cell migration within a natural context is tightly controlled, often by specific transcription factors. However, the switch from stationary to migratory behavior is poorly understood. Border cells perform a spatially and temporally controlled invasive migration during Drosophila oogenesis. Slbo, a C/EBP family transcriptional activator, is required for them to become migratory. We purified wild-type and slbo mutant border cells as well as nonmigratory follicle cells and performed comparative whole-genome expression profiling, followed by functional tests of the contributions of identified targets to migration. About 300 genes were significantly upregulated in border cells, many dependent on Slbo. Among these, the microtubule regulator Stathmin was strongly upregulated and was required for normal migration. Actin cytoskeleton regulators were also induced, including, surprisingly, a large cluster of "muscle-specific" genes. We conclude that Slbo induces multiple cytoskeletal effectors, and that each contributes to the behavioral changes in border cells.
AB - Cell migration within a natural context is tightly controlled, often by specific transcription factors. However, the switch from stationary to migratory behavior is poorly understood. Border cells perform a spatially and temporally controlled invasive migration during Drosophila oogenesis. Slbo, a C/EBP family transcriptional activator, is required for them to become migratory. We purified wild-type and slbo mutant border cells as well as nonmigratory follicle cells and performed comparative whole-genome expression profiling, followed by functional tests of the contributions of identified targets to migration. About 300 genes were significantly upregulated in border cells, many dependent on Slbo. Among these, the microtubule regulator Stathmin was strongly upregulated and was required for normal migration. Actin cytoskeleton regulators were also induced, including, surprisingly, a large cluster of "muscle-specific" genes. We conclude that Slbo induces multiple cytoskeletal effectors, and that each contributes to the behavioral changes in border cells.
UR - http://www.scopus.com/inward/record.url?scp=33645531742&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2006.02.004
DO - 10.1016/j.devcel.2006.02.004
M3 - Article
C2 - 16580994
AN - SCOPUS:33645531742
SN - 1534-5807
VL - 10
SP - 497
EP - 508
JO - Developmental cell
JF - Developmental cell
IS - 4
ER -