Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.

C. T. Lutz, W. C. Hollifield, B. Seed, J. M. Davie, H. V. Huang

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.

Original languageEnglish
Pages (from-to)4379-4383
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number13
DOIs
StatePublished - Jul 1987
Externally publishedYes

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