TY - JOUR
T1 - Syrinx 2A
T2 - an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.
AU - Lutz, C. T.
AU - Hollifield, W. C.
AU - Seed, B.
AU - Davie, J. M.
AU - Huang, H. V.
PY - 1987/7
Y1 - 1987/7
N2 - The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.
AB - The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.
UR - http://www.scopus.com/inward/record.url?scp=0023374445&partnerID=8YFLogxK
U2 - 10.1073/pnas.84.13.4379
DO - 10.1073/pnas.84.13.4379
M3 - Article
C2 - 2955406
AN - SCOPUS:0023374445
SN - 0027-8424
VL - 84
SP - 4379
EP - 4383
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -