SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae

Paul Cliften; Yeelan Wang; Daisuke Mochizuki; Tokichi Miyakawa; Rungrach Wangspa; Joanne Hughes; Jon Y. Takemoto

doi:10.1099/13500872-142-3-477

SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae

Paul Cliften, Yeelan Wang, Daisuke Mochizuki, Tokichi Miyakawa, Rungrach Wangspa, Joanne Hughes, Jon Y. Takemoto

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Abstract

The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.

Original language	English
Pages (from-to)	477-484
Number of pages	8
Journal	Microbiology
Volume	142
Issue number	3
DOIs	https://doi.org/10.1099/13500872-142-3-477
State	Published - Mar 1996

Keywords

Phospholipid biosynthesis
SUR2
SYR2
Saccharomyces cerevisiae
Syringomycin

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abstract = "The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.",

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T1 - SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae

AU - Cliften, Paul

AU - Wang, Yeelan

AU - Mochizuki, Daisuke

AU - Miyakawa, Tokichi

AU - Wangspa, Rungrach

AU - Hughes, Joanne

AU - Takemoto, Jon Y.

PY - 1996/3

Y1 - 1996/3

N2 - The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.

AB - The Pseudomonas syringae cyclic lipodepsipeptide syringomycin inhibits the growth of Saccharomyces cerevisiae. A novel yeast gene, SYR2, was found to complement two syringomycin-resistant S. cerevisiae mutants. SYR2 was cloned, sequenced, and shown to encode a 349 amino acid protein located in the endoplasmic reticulum. SYR2 was identical to SUR2, which is involved in survival during nutritional starvation. Gene disruption or overexpression of SYR2 did not affect cell viability or ergosterol levels, but did influence cellular phospholipid levels. The findings suggest that phospholipids are important for the growth inhibitory action of syringomycin.

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KW - Syringomycin

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SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae

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