Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy

Yang Pu, Rui Tang, Jianpeng Xue, W. B. Wang, Baogang Xu, Duanwen Shen, Sharon Bloch, Mingzhou Zhou, S. Achilefu, R. R. Alfano

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The clinical diagnosis of most cancers is based on evaluation of histology microscopic slide to view the size and shape of cellular nuclei, and morphological structure of tissue. To achieve this goal in vivo and in deep tissue, near infrared (NIR) dyes-bovine serum albumin (BSA) and immunoglobulin G (IgG) conjugates were synthesized. The spectral study show that the absorption and fluorescence of the dye-conjugates are in the "tissue optical window" between 650 nm and 1100 nm. The internalization and pinocytosis of the synthesized compound were investigated in cell level using fluorescence microscopy to obtain the optimal concentration and staining time scale.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
DOIs
StatePublished - 2013
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI - San Francisco, CA, United States
Duration: Feb 2 2013Feb 5 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8587
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/2/1302/5/13

Keywords

  • Near infrared (NIR)
  • dye-bioconjugates
  • fluorescence microscopy
  • prostate cancer cell lines

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