Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system

Alan Shiels, Ian Phillips, Stephen Jeffrey, Elizabeth Shephard, Nicholas Carter

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS-polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.

Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalFEBS Letters
Volume148
Issue number1
DOIs
StatePublished - Nov 1 1982

Keywords

  • Carbonic anhydrase
  • Immunoprecipitation
  • Muscle
  • Radioimmunoassay
  • Translation in vitro
  • mRNA

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