Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system

Alan Shiels; Ian Phillips; Stephen Jeffrey; Elizabeth Shephard; Nicholas Carter

doi:10.1016/0014-5793(82)81256-8

Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system

Alan Shiels, Ian Phillips, Stephen Jeffrey, Elizabeth Shephard, Nicholas Carter

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS-polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same M_r-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.

Original language	English
Pages (from-to)	122-126
Number of pages	5
Journal	FEBS Letters
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(82)81256-8
State	Published - Nov 1 1982

Keywords

Carbonic anhydrase
Immunoprecipitation
Muscle
Radioimmunoassay
Translation in vitro
mRNA

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10.1016/0014-5793(82)81256-8

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abstract = "Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS-polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.",

keywords = "Carbonic anhydrase, Immunoprecipitation, Muscle, Radioimmunoassay, Translation in vitro, mRNA",

author = "Alan Shiels and Ian Phillips and Stephen Jeffrey and Elizabeth Shephard and Nicholas Carter",

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T1 - Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system

AU - Shiels, Alan

AU - Phillips, Ian

AU - Jeffrey, Stephen

AU - Shephard, Elizabeth

AU - Carter, Nicholas

PY - 1982/11/1

Y1 - 1982/11/1

N2 - Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS-polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.

AB - Carbonic anhydrase III (CA III) was identified in the products of rat soleus muscle RNA translation in vitro by both a radioimmunoassay and a specific immunoprecipitation technique followed by SDS-polyacrylamide gel electrophoresis analysis of the precipitated antigen. The primary translation product has the same Mr-value as the native isoenzyme. CA III mRNA was found to represent ∼0.55% of the total mRNA present in rat soleus muscle.

KW - Carbonic anhydrase

KW - Immunoprecipitation

KW - Muscle

KW - Radioimmunoassay

KW - Translation in vitro

KW - mRNA

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DO - 10.1016/0014-5793(82)81256-8

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AN - SCOPUS:0020211944

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JF - FEBS Letters

IS - 1

ER -

Synthesis of rat muscle carbonic anhydrase III in a cell-free translation system

Abstract

Keywords

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