Synthesis of oltgonucleotides with sequences identical with or analogous to the 3′-end of 16S ribosomal RNA of es cherichia coli: Preparation of m62A-C-C-U-C-C and A-C-C-U-C-m42C via phosphotriester intermediates

J. H. van Boom, P. M.J. Burgers, R. Crea, G. Van Der Marel, G. Wille

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Abstract

The synthesis of two fully-protected hexanucleotides [11a and 11b] via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 57′-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3′→5′ internucleotide linkages.

Original languageEnglish
Pages (from-to)747-759
Number of pages13
JournalNucleic acids research
Volume4
Issue number3
DOIs
StatePublished - Mar 1977

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