@inbook{4ae583e0d8944315b614b26346695e2a,
title = "Synthesis and Expression of a Targeted, Ferritin-Based Tracer for PET Imaging of Kidney Glomeruli",
abstract = "Cationic ferritin (CF) has been developed as a multimodal, targeted imaging tracer to directly detect and map nephrons in the kidney in vivo. Direct detection of functional nephrons provides a unique, sensitive biomarker to predict or monitor kidney disease progression. CF has been developed to map functional nephron number from magnetic resonance imaging (MRI) or positron emission tomography (PET). Previous preclinical imaging studies have used non-human-derived ferritin and commercial formulations that must still be developed for translation to clinical use. Here we describe the reproducible formulation of CF (either derived from horse or from human recombinant ferritin) optimized for intravenous injection and radiolabeling by PET. The human recombinant heteropolymer ferritin is spontaneously assembled in liquid culture (Escherichia coli, E. coli) and modified to form human recombinant cationic ferritin (HrCF) to mitigate potential immunologic reactions for use in humans.",
keywords = "Cationic ferritin, Human recombinant ferritin, Magnetic resonance imaging, Nephron mass, Positron emission tomography",
author = "Baldelomar, \{Edwin J.\} and Emoto, \{Kasey C.\} and Jordan, \{Maria Veronica Clavijo\} and Charlton, \{Jennifer R.\} and Reichert, \{David E.\} and Neda Parvin and Courtnie Yokono and Hann, \{Bradley D.\} and Bennett, \{Kevin M.\}",
note = "Funding Information: We gratefully acknowledge the Preclinical PET Imaging Facility and Biomedical Magnetic Resonance Center at Washington University School of Medicine in St. Louis. This work was supported by National Institutes of Health (NIH) grants R01DK110622, R01DK111861, and R41DK129138. E. J. Baldelomar also received support from NIH grant TL1TR002344. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3179-9\_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "201--213",
booktitle = "Methods in Molecular Biology",
}