Synthesis and evaluation of an 18F-labeled pyrimidine-pyridine amine for targeting CXCR4 receptors in gliomas

Dustin Wayne Demoin, Masahiro Shindo, Hanwen Zhang, Kimberly J. Edwards, Inna Serganova, Naga Vara Kishore Pillarsetty, Jason S. Lewis, Ronald G. Blasberg

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Introduction Chemokine receptor-4 (CXCR4, fusin, CD184) is expressed on several tissues involved in immune regulation and is upregulated in many diseases including malignant gliomas. A radiolabeled small molecule that readily crosses the blood–brain barrier can aid in identifying CXCR4-expressing gliomas and monitoring CXCR4-targeted therapy. In the current work, we have synthesized and evaluated an [18F]-labeled small molecule based on a pyrimidine–pyridine amine for its ability to target CXCR4. Experimental The nonradioactive standards and the nitro precursor used in this study were prepared using established methods. An HPLC method was developed to separate the nitro-precursor from the nonradioactive standard and radioactive product. The nitro-precursor was radiolabeled with 18F under inert, anhydrous conditions using the [18F]-kryptofix 2.2.2 complex to form the desired N-(4-(((6-[18F]fluoropyridin-2-yl)amino)methyl)benzyl)pyrimidin-2-amine ([18F]-3). The purified radiolabeled compound was used in serum stability, partition coefficient, cellular uptake, and in vivo cancer targeting studies. Results [18F]-3 was synthesized in 4–10% decay-corrected yield (to start of synthesis). [18F]-3 (tR ≈ 27 min) was separated from the precursor (tR ≈ 30 min) using a pentafluorophenyl column with an isocratic solvent system. [18F]-3 displayed acceptable serum stability over 2 h. The amount of [18F]-3 bound to the plasma proteins was determined to be > 97%. The partition coefficient (LogD7.4) is 1.4 ± 0.5. Competitive in vitro inhibition indicated 3 does not inhibit uptake of 67Ga-pentixafor. Cell culture media incubation and ex vivo urine analysis indicate rapid metabolism of [18F]-3 into hydrophilic metabolites. Thus, in vitro uptake of [18F]-3 in CXCR4 overexpressing U87 cells (U87 CXCR4) and U87 WT indicated no specific binding. In vivo studies in mice bearing U87 CXCR4 and U87 WT tumors on the left and right shoulders were carried out using [18F]-3 and 68Ga-pentixafor on consecutive days. The CXCR4 positive tumor was clearly visualized in the PET study using 68Ga-pentixafor, but not with [18F]-3. Conclusions We have successfully synthesized both a radiolabeled analog to previously reported CXCR4-targeting molecules and a nitro precursor. Our in vitro and in vivo studies indicate that [18F]-3 is rapidly metabolized and, therefore, does not target CXCR4-expressing tumors. Optimization of the structure to improve the in vivo (and in vitro) stability, binding, and solubility could lead to an appropriate CXCR4-targeted radiodiagnositic molecule.

Original languageEnglish
Pages (from-to)606-611
Number of pages6
JournalNuclear Medicine and Biology
Issue number10
StatePublished - Oct 1 2016


  • CXCR4
  • Dipyrimidine amine
  • F
  • Pyrimidine-pyridine amine
  • Radiosynthesis


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