TY - JOUR
T1 - Synthesis and Characterization of Injectable Sulfonate-Containing Hydrogels
AU - Liang, Jue
AU - Karakoçak, Bedia Begüm
AU - Struckhoff, Jessica J.
AU - Ravi, Nathan
N1 - Funding Information:
This research was supported by the NIH Grant EY021620 and Department of Veterans Affairs Rehab Merit Review Grant RX000657-01 to N.R. Also, Research to Prevent Blindness, Inc. and NIH Core Grant P30 EY02687 facilities supported parts of the research. We thank Mr. Paul Hamilton for conducting the GPC test.
Publisher Copyright:
© 2016 American Chemical Society
PY - 2016/12/12
Y1 - 2016/12/12
N2 - Sulfonate-containing hydrogels are of particular interest because of their tunable mechanical and swelling properties, as well as their biological effects. Polysulfonate copolymers were synthesized by reacting 2-acrylamido-2-methylpropanesulfonic acid (AMPS), acrylamide (AM), and acrylic acid (AA). We found that the incorporation rate of sulfonate-containing monomer and the molecular weight of the copolymer were significantly enhanced by increasing the ionic strength of the solution. We introduced thiol groups by modifying the pendant carboxylates or copolymerizing along with a disulfide-containing monomer. The thiol-containing copolymers were reacted with a 4-arm acrylamide-terminated poly(ethylene glycol) via a thiol-ene click reaction, which was mediated by a photoinitiator, a redox initiator, or a base-catalyzed Michael-Addition. We were able to tailor the storage modulus (33-1800 Pa) and swelling capacity (1-91 wt %) of the hydrogel by varying the concentration of the copolymers. We determined that the injectable sulfonate-containing hydrogels were biocompatible up to 20 mg/mL, as observed by an electric cell-substrate impedance sensing (ECIS) technique, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using three different cell lines: human retinal pigment epithelial cells (ARPE-19), fibroblasts (NIH 3T3), and Chinese hamster ovary cells (CHO).
AB - Sulfonate-containing hydrogels are of particular interest because of their tunable mechanical and swelling properties, as well as their biological effects. Polysulfonate copolymers were synthesized by reacting 2-acrylamido-2-methylpropanesulfonic acid (AMPS), acrylamide (AM), and acrylic acid (AA). We found that the incorporation rate of sulfonate-containing monomer and the molecular weight of the copolymer were significantly enhanced by increasing the ionic strength of the solution. We introduced thiol groups by modifying the pendant carboxylates or copolymerizing along with a disulfide-containing monomer. The thiol-containing copolymers were reacted with a 4-arm acrylamide-terminated poly(ethylene glycol) via a thiol-ene click reaction, which was mediated by a photoinitiator, a redox initiator, or a base-catalyzed Michael-Addition. We were able to tailor the storage modulus (33-1800 Pa) and swelling capacity (1-91 wt %) of the hydrogel by varying the concentration of the copolymers. We determined that the injectable sulfonate-containing hydrogels were biocompatible up to 20 mg/mL, as observed by an electric cell-substrate impedance sensing (ECIS) technique, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using three different cell lines: human retinal pigment epithelial cells (ARPE-19), fibroblasts (NIH 3T3), and Chinese hamster ovary cells (CHO).
UR - http://www.scopus.com/inward/record.url?scp=85006132456&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.6b01368
DO - 10.1021/acs.biomac.6b01368
M3 - Article
C2 - 27936721
AN - SCOPUS:85006132456
SN - 1525-7797
VL - 17
SP - 4064
EP - 4074
JO - Biomacromolecules
JF - Biomacromolecules
IS - 12
ER -