@article{a16d221aa62d4d1a8651eb1d52374599,
title = "Synergistic and tunable human gene activation by combinations of synthetic transcription factors",
abstract = "Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.",
author = "Pablo Perez-Pinera and Ousterout, {David G.} and Brunger, {Jonathan M.} and Farin, {Alicia M.} and Glass, {Katherine A.} and Farshid Guilak and Crawford, {Gregory E.} and Hartemink, {Alexander J.} and Gersbach, {Charles A.}",
note = "Funding Information: This work was supported by a US National Institutes of Health (NIH) Director{\textquoteright}s New Innovator Award (DP2-OD008586), a National Science Foundation (NSF) Faculty Early Career Development (CAREER) Award (CBET-1151035), NIH R03-AR061042, The Hartwell Foundation Individual Biomedical Research Award and a March of Dimes Basil O{\textquoteright}Connor Starter Scholar Award to C.A.G.; grants from the NIH (P50-GM081883) and the Defense Advance Research Projects Agency (HR0011-09-1-0040) to A.J.H.; and grants from the NIH to G.E.C. (U54-HG004563) and F.G. (R01-AR048852). D.G.O. was supported by a predoctoral fellowship from the American Heart Association. K.A.G. was supported by an NSF Graduate Research Fellowship.",
year = "2013",
month = mar,
doi = "10.1038/nmeth.2361",
language = "English",
volume = "10",
pages = "239--242",
journal = "Nature Methods",
issn = "1548-7091",
number = "3",
}