@article{ea7bfbb04f2748feb11ec82aeb1e2ed0,
title = "Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO₂",
abstract = "Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source, and CO₂, a greenhouse gas. Important traits for such microbes are fast growth and amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, a unicellular cyanobacterium capable of rapid autotrophic growth, comparable to heterotrophic industrial hosts such as yeast. Synechococcus UTEX 2973 can be readily transformed for facile generation of desired knockout and knock-in mutations. Genome sequencing coupled with global proteomics studies revealed that Synechococcus UTEX 2973 is a close relative of the widely studied cyanobacterium Synechococcus elongatus PCC 7942, an organism that grows more than two times slower. A small number of nucleotide changes are the only significant differences between the genomes of these two cyanobacterial strains. Thus, our study has unraveled genetic determinants necessary for rapid growth of cyanobacterial strains of significant industrial potential.",
author = "Jingjie Yu and Michelle Liberton and Cliften, {Paul F.} and Head, {Richard D.} and Jacobs, {Jon M.} and Smith, {Richard D.} and Koppenaal, {David W.} and Brand, {Jerry J.} and Pakrasi, {Himadri B.}",
note = "Funding Information: We thank all members of the Pakrasi lab for helpful discussions, and Howard Berg at the Integrated Microscopy Facility of Donald Danforth Plant Science Center for TEM assistance. Work at Washington University was primarily supported by U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research. The electron microscopy study was supported as part of the Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035. LC-MS/MS proteomics work was performed in the Environmental Molecular Sciences Laboratory, a U. S. Department of Energy Office of Biological and Environmental Research national scientific user facility located at Pacific Northwest National Laboratory in Richland, Washington. Pacific Northwest National Laboratory is operated by Battelle for the U.S. Department of Energy under Contract No. DE-AC05-76RLO 1830. Work at University of Texas at Austin was supported by the National Science Foundation under Award number DBI-1201881.",
year = "2015",
doi = "10.1038/srep08132",
language = "English",
volume = "5",
pages = "8132",
journal = "Scientific Reports",
issn = "2045-2322",
}