@article{4f9fff431c724de5991815d1b64f7b4c,
title = "SYD-2 Liprin-α organizes presynaptic active zone formation through ELKS",
abstract = "A central event in synapse development is formation of the presynaptic active zone in response to positional cues. Three active zone proteins, RIM, ELKS (also known as ERC or CAST) and Liprin-α, bind each other and are implicated in linking active zone formation to synaptic vesicle release. Loss of function in Caenorhabditis eleganssyd-2 Liprin-α alters the size of presynaptic specializations and disrupts synaptic vesicle accumulation. Here we report that a missense mutation in the coiled-coil domain of SYD-2 causes a gain of function. In HSN synapses, the syd-2(gf) mutation promotes synapse formation in the absence of syd-1, which is essential for HSN synapse formation. syd-2(gf) also partially suppresses the synaptogenesis defects in syg-1 and syg-2 mutants. The activity of syd-2(gf) requires elks-1, an ELKS homolog; but not unc-10, a RIM homolog. The mutant SYD-2 shows increased association with ELKS. These results establish a functional dependency for assembly of the presynaptic active zone in which SYD-2 plays a key role.",
author = "Ya Dai and Hidenori Taru and Deken, {Scott L.} and Brock Grill and Brian Ackley and Nonet, {Michael L.} and Yishi Jin",
note = "Funding Information: We thank K. Shen (Stanford University) for kyIs235 marker, Punc-86GFP plasmid and for communicating unpublished data, A.D. Chisholm for advice on genetics and statistic analysis, the National Bioresource project for the Experimental Animal C. elegans for elks-1(tm1233), T. Ishihara (Kyushu University) for VenusGFP vector, J. McEwan and J. Kaplan (Massachusetts General Hospital) for Pttx-3-RFP and Pttx-3-GFP plasmids, and the members of the Jin and Chisholm labs for discussions. This work was supported by grants from the US National Institutes of Health to Y.J. and M.N., postdoctoral fellowships from Japanese Society for Promoting Science and the Uehara Memorial Foundation to H.T., a Canadian CIHR fellowship to B.G., and a Career Development Award from the Muscular Dystrophy Association to B.A. Y.D. is an research associate, and Y.J. is an investigator, of the Howard Hughes Medical Institute.",
year = "2006",
month = dec,
doi = "10.1038/nn1808",
language = "English",
volume = "9",
pages = "1479--1487",
journal = "Nature neuroscience",
issn = "1097-6256",
number = "12",
}