Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

Hong Niu; Haichang Li; Ya Guan; Xin Zhou; Zhongguang Li; Serana Li Zhao; Peng Chen; Tao Tan; Hua Zhu; Valerie Bergdall; Xuehong Xu; Jianjie Ma; Jianjun Guan

doi:10.1016/j.bioactmat.2022.03.017

Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

Hong Niu
, Haichang Li
, Ya Guan
, Xin Zhou
, Zhongguang Li
, Serana Li Zhao
, Peng Chen
, Tao Tan
, Hua Zhu
, Valerie Bergdall
, Xuehong Xu
, Jianjie Ma
, Jianjun Guan

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53^−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.

Original language	English
Pages (from-to)	104-115
Number of pages	12
Journal	Bioactive Materials
Volume	18
DOIs	https://doi.org/10.1016/j.bioactmat.2022.03.017
State	Published - Dec 2022

Keywords

Controlled drug delivery
Diabetic wound healing
MG53
ROS-Scavenging hydrogel
hair follicle stem cell

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10.1016/j.bioactmat.2022.03.017

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title = "Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development",

abstract = "MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.",

keywords = "Controlled drug delivery, Diabetic wound healing, MG53, ROS-Scavenging hydrogel, hair follicle stem cell",

author = "Hong Niu and Haichang Li and Ya Guan and Xin Zhou and Zhongguang Li and Zhao, \{Serana Li\} and Peng Chen and Tao Tan and Hua Zhu and Valerie Bergdall and Xuehong Xu and Jianjie Ma and Jianjun Guan",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = dec,

doi = "10.1016/j.bioactmat.2022.03.017",

language = "English",

volume = "18",

pages = "104--115",

journal = "Bioactive Materials",

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T1 - Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

AU - Niu, Hong

AU - Li, Haichang

AU - Guan, Ya

AU - Zhou, Xin

AU - Li, Zhongguang

AU - Zhao, Serana Li

AU - Chen, Peng

AU - Tan, Tao

AU - Zhu, Hua

AU - Bergdall, Valerie

AU - Xu, Xuehong

AU - Ma, Jianjie

AU - Guan, Jianjun

PY - 2022/12

Y1 - 2022/12

N2 - MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.

AB - MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.

KW - Controlled drug delivery

KW - Diabetic wound healing

KW - MG53

KW - ROS-Scavenging hydrogel

KW - hair follicle stem cell

UR - https://www.scopus.com/pages/publications/85126536532

U2 - 10.1016/j.bioactmat.2022.03.017

DO - 10.1016/j.bioactmat.2022.03.017

M3 - Article

AN - SCOPUS:85126536532

SN - 2452-199X

VL - 18

SP - 104

EP - 115

JO - Bioactive Materials

JF - Bioactive Materials

ER -

Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

Abstract

Keywords

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